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首页> 外文期刊>Journal of Medical Entomology >Use of enzyme-linked immunosorbent assay for detection of natural leishmania infections in phlebotomine sand flies from southeastern Turkey
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Use of enzyme-linked immunosorbent assay for detection of natural leishmania infections in phlebotomine sand flies from southeastern Turkey

机译：酶联免疫吸附法用于检测土耳其东南部毒蛇毒沙蝇中天然利什曼原虫感染的方法

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摘要

In total, 320 phelebotomine sand flies from Alibozlu (Osmaniye), Kizyusuflu (Osmaniye), and Sanliurfa in southeastern Turkey were tested for the detection and identification of Leishmania in vector sand flies by enzyme-linked immunosorbent assay with species-specific monoclonal antibodies. We used monoclonal antibodies that recognize both Leishmania tropica and Leishmania major, and a monoclonal antibody specific only to L. tropica. Phosphate-buffered saline and monoclonal antibody M2 recognizing Leishmania amazonensis were used as controls. Infection rates of sand flies were 0.9% in Alibozlu, 0% Kizyusuflu, and 3.6% in Sanliurfa. Positive sand flies were identified as Phlebotomus sergenti Parrot and Phlebotomus major syriacus Adler & Theodor.
机译：通过物种特异性单克隆抗体的酶联免疫吸附试验，总共对土耳其东南部的阿里博兹卢（Osmaniye），克孜苏苏弗（Osmaniye）和Sanliurfa的320苯乙胺嘧啶沙蝇进行了检测，以检测和鉴定载体沙蝇中的利什曼原虫。我们使用了识别热带利什曼原虫和大利什曼原虫的单克隆抗体，以及仅对热带利什曼原虫具有特异性的单克隆抗体。磷酸缓冲盐溶液和识别亚马逊利什曼原虫的单克隆抗体M2用作对照。沙蝇的感染率在Alibozlu为0.9％，Kizyusuflu为0％，在Sanliurfa为3.6％。阳性沙蝇被鉴定为大白尾鹦鹉鹦鹉和大白斑潜蝇sylercus Adler＆Theodor。

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