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首页> 外文期刊>Journal of Medical Entomology >DNA barcodes can distinguish species of Indian mosquitoes (Diptera: Culicidae)
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DNA barcodes can distinguish species of Indian mosquitoes (Diptera: Culicidae)

机译：DNA条形码可以区分印度蚊子的种类（双翅目：葫芦科）

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摘要

Species identification of mosquitoes (Diptera: Culicidae) based on morphological characteristics remains often difficult in field-collected mosquito specimens in vector-borne disease surveillance programs. The use of DNA barcodes has been proposed recently as a tool for identification of the species in many diverse groups of animals. However, the efficacy of this tool for mosquitoes remains unexplored. Hence, a study was undertaken to construct DNA barcodes for several species of mosquitoes prevalent in India, which included major vector species. In total, 111 specimens of mosquitoes belonging to 15 genera, morphologically identified to be 63 species, were used. This number also included multiple specimens for 22 species. DNA barcode approach based on DNA sequences of mitochondrial cytochrome oxidase gene sequences could identify 62 species among these, in confirmation with the conventional taxonomy. However, two closely related species, Ochlerotatus portonovoensis (Tiwari & Hiriyan) and Ochlerotatus wardi (Reinert) could not be identified as separate species based on DNA barcode approach, their lineages indicating negligible genetic divergence (Kimura two-parameter genetic distance = 0.0043).
机译：在媒介传播的疾病监测计划中，在野外采集的蚊子标本中，根据形态学特征识别蚊子（双翅目：Culicidae）的种类仍然很困难。最近已经提出使用DNA条形码作为在许多不同动物组中鉴定物种的工具。但是，这种工具对蚊子的功效仍待探索。因此，进行了一项研究以构建针对印度流行的几种蚊子的DNA条码，其中包括主要的媒介种。总共使用了15个属的111个蚊子标本，从形态上鉴定为63种。这个数字还包括22种的多个标本。基于线粒体细胞色素氧化酶基因序列的DNA序列的DNA条形码方法可以识别其中的62种，这与常规分类法相符。然而，基于DNA条码方法，不能将两个密切相关的物种Portoovoensis Ochlerotatus portonovoensis（Tiwari和Hiriyan）和Warch Ochlerotatus wardi（Reinert）识别为单独的物种，它们的谱系表明遗传差异可忽略不计（Kimura两参数遗传距离= 0.0043）。

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