首页> 外文期刊>Journal of Medical Entomology >CNAct-1 gene is differentially expressed in the subtropical mosquito Culex nigripalpus (Diptera: Culicidae), the primary West Nile Virus vector in Florida.
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CNAct-1 gene is differentially expressed in the subtropical mosquito Culex nigripalpus (Diptera: Culicidae), the primary West Nile Virus vector in Florida.

机译:CNAct-1基因在亚热带蚊子库蚊(Dilexera:Culicidae)(佛罗里达州的主要西尼罗河病毒载体)中差异表达。

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摘要

Analysis of differentially expressed genes is a common molecular biological tool to investigate changes in mosquito genes after a bloodmeal or parasite exposure. We report here the characterization of a differentially expressed actin gene, CNAct-1, from the subtropical mosquito, Culex nigripalpus Theobald (Diptera: Culicidae). The CNAct-1 genomic clone is 1.525 kb, includes one 66-bp intron, and a 328-bp 3'-untranslated region. The 376-amino acid putative translation product shares high similarity with muscle-specific actin proteins from other insects, including Culex pipiens pipiens L., Aedes aegypti (L.), Anopheles gambiae Giles and Drosophila melanogaster (Meigen). CNAct-1 is expressed in second and third instars, late pupae, and adult females and males. Interestingly, Cx. nigripalpus actin was highly expressed in female mosquito midgut tissue isolated 6-12 h after ingestion of a bloodmeal. This expression profile indicates a unique function for CNAct-1 in midgut processes that are initiated after blood ingestion.
机译:差异表达基因的分析是调查血粉或寄生虫暴露后蚊子基因变化的常见分子生物学工具。我们在这里报告了来自亚热带蚊子库蚊(Nilexalgripalpus Theobald)(双翅目:库蚊科)的差异表达肌动蛋白基因CNAct-1的表征。 CNAct-1基因组克隆为1.525 kb,包括一个66 bp的内含子和一个328 bp的3'非翻译区。 376个氨基酸的推定翻译产物与其他昆虫的肌肉特异性肌动蛋白具有高度相似性,这些昆虫包括库蚊(Pilexens pipiens pipiens L.),埃及伊蚊(Aedes aegypti)(L.),冈比亚按蚊(Anopheles gambiae Giles)和果蝇(Drosophila melanogaster)(Meigen)。 CNAct-1在第二和第三龄幼虫,late中以及成年雌性和雄性中表达。有趣的是,Cx。摄入血粉后6至12小时,在分离的雌性蚊子中肠组织中,黑头手肌肌动蛋白高表达。该表达谱表明在血液摄入后启动的中肠过程中CNAct-1的独特功能。

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