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首页> 外文期刊>Journal of Medical Entomology >Molecular analysis of a para sodium channel gene from pyrethroid-resistant head lice, Pediculus humanus capitis (Anoplura : Pediculidae)
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Molecular analysis of a para sodium channel gene from pyrethroid-resistant head lice, Pediculus humanus capitis (Anoplura : Pediculidae)

机译：拟除虫菊酯抗性头虱对虾钠通道基因的分子分析（Anoplura：Pediculidae）

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摘要

The problem of pyrethroid-resistance in bead lice, Pediculus humanus capitis (De Geer), is growing worldwide, and an insensitive sodium channel is suspected as the major mechanism of this resistance. We sequenced an open reading frame (ORF) encoding for the para-orthologous sodium channel from an insecticide-susceptible strain of the body louse, Pediculus humanus humanus (L.), based on conserved peptide sequences and a known partial gene sequence. Phenothrin-susceptible and -resistant head louse colonies from Japanese were individually analyzed for point mutations of the sodium channel cDNA; susceptible bead and body lice differed in double homozygous synonymous substitutions. The resistant bead lice shared 23 base substitutions homozygously, in which four resulted in amino acid substitutions: D11E in the N-terminal inner-membrane segment; M850T in the outer-membrane loop between segments four and five of domain II; T952I and L955 F in the trans-membrane segment five of domain II. The latter two substitutions coincided with those of pyrethroid-resistant head lice in the U.S. and U.K. (Lee et al. 2000), within the available published information on the peptide sequences. The potential mechanisms of head louse pyrethroid-resistance are discussed based on the four structural changes of the target molecule.
机译：珠虱虱子拟除虫菊酯的抗药性问题在世界范围内日益扩大，怀疑不敏感的钠通道是这种抗药性的主要机理。我们基于保守的肽序列和已知的部分基因序列，对编码自体虱的易感杀虫剂的人对拟南芥钠通道的开放阅读框（ORF）进行了测序。分别分析了日本人对苯乙菊酯敏感和耐药的头虱菌落中钠通道cDNA的点突变。易感珠和体虱在双重纯合同义替换上有所不同。抗性珠虱纯合地共有23个碱基取代，其中四个导致氨基酸取代：N末端内膜片段中的D11E;域II的第4段和第5段之间的外膜环路中的M850T;在域II的跨膜片段5中的T952I和L955F。在有关肽序列的可获得的公开信息中，后两种取代与美国和英国的拟除虫菊酯抗性头虱的取代（Lee et al。2000）一致。基于靶分子的四个结构变化，探讨了对头虱拟除虫菊酯抗性的潜在机制。

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