• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Medical Entomology >Experimental infection of California birds with western equine encephalomyelitis and St. Louis encephalitis viruses
【24h】

Experimental infection of California birds with western equine encephalomyelitis and St. Louis encephalitis viruses

机译：实验性感染加利福尼亚鸟类的西部马脑脊髓炎和圣路易斯脑炎病毒

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A total of 27 bird species from the San Joaquin and Coachella valleys of California were inoculated subcutaneously with sympatric strains of western equine encephalomyelitis (WEE) and St. Louis encephalitis (SLE) viruses. Overall, 133 of 164 birds inoculated with WEE virus developed a viremia detected by plaque assay; significantly greater than 72 of 163 birds inoculated with SLE virus. Host competence was calculated as the average number of days that each avian species had a viremia greater than or equal to 2 log(10) plaque-forming units per 0.1 ml, the threshold for infecting susceptible Culex tarsalis Coquillett, the primary vector of these viruses in California. Eleven of 20 species inoculated with WEE virus had a value greater than or equal to 1 and were considered to be competent hosts, whereas only six of 22 species inoculated with SLE virus had a value greater than or equal to 1. Overall, 133 of 164 birds inoculated with WEE virus and 105 of 163 inoculated with SLE virus produced antibody detectable by enzyme immunoassay and/or plaque reduction neutralization test. Six birds infected with WEE virus (one house finch, three mourning doves, one Brewer's sparrow, and one white-crowned sparrow) and nine birds infected with SLE virus (two house finches, three white-crowned sparrows, one song sparrow, two Western scrub-jays, and one orange crowned warbler) contained viral RNA detected by reverse transcription-polymerase chain reaction at necropsy > 6 wk postinoculation; infectious WEE and SLE viruses were only recovered from three mourning doves and an orange-crowned warbler, respectively, after blind passage in mosquito cells. Our study indicated that birds with elevated field antibody prevalence rates may not be the most competent hosts for encephalitis viruses and that relatively few birds developed chronic infections that could be important in virus persistence and dispersal.
机译：皮下接种了来自加利福尼亚州圣华金和科切拉山谷的总共27种鸟类皮下接种了西马脑脊髓炎（WEE）和圣路易斯脑炎（SLE）病毒的同伴株。总体而言，接种WEE病毒的164只鸡中有133只通过斑块检测发现了病毒血症。大大超过了SLE病毒接种的163只禽中的72只。宿主能力的计算公式为：每个禽类病毒的毒血症天数大于或等于每0.1毫升2个log（10）噬菌斑形成单位，这是感染易感库克斯lex鱼Coquillett（这些病毒的主要载体）的阈值在加利福尼亚。接种WEE病毒的20个物种中，有11个的值大于或等于1，并被认为是合格宿主，而接种SLE病毒的22个物种中，只有6个的值大于或等于1。总体而言，164个中的133个接种WEE病毒的鸟类和163接种SLE病毒的鸟类中有105株可通过酶免疫测定和/或噬菌斑减少中和试验检测到抗体。六只感染WEE病毒的鸟（一只雀科，三只哀悼的鸽子，一只布鲁尔的麻雀和一只白冠麻雀）和九只被SLE病毒感染的鸟（两只雀科，三只白冠麻雀，一只鸣麻雀，两只西尸检> 6 wk接种后，通过逆转录-聚合酶链反应检测到病毒RNA。在蚊子细胞中盲目通过后，仅分别从三只哀悼的鸽子和一只橘黄色的鸣鸟中回收了感染性WEE和SLE病毒。我们的研究表明，具有较高的现场抗体流行率的鸟类可能不是脑炎病毒的最有力宿主，而且相对较少的鸟类出现了慢性感染，这可能对病毒的持久性和传播很重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号