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首页> 外文期刊>Journal of Medical Entomology >An automated feeding apparatus for in vitro maintenance of the human head louse, Pediculus capitis (Anoplura : Pediculidae)
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An automated feeding apparatus for in vitro maintenance of the human head louse, Pediculus capitis (Anoplura : Pediculidae)

机译：用于人头虱体外培养的自动饲喂设备（无头虱：Pediculidae）

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摘要

An automated feeding apparatus was developed to maintain the human head louse (Pediculus capitis DeGeer) in vitro. With the use of valves and timers, banked human blood and saline from refrigerated reservoirs were pumped into and flushed out of the system every 7 d. During this rotational interval, bloodmeals were provided to head lice continuously and ad libitum through a stretched Nescofilm-silicone sandwich membrane. Compared with our previous in vitro human head louse-rearing apparatus, greater numbers of lice could be fed simultaneously with minimal human monitoring. Development of second to third instars and third instars to adults was significantly faster when lice were reared in vivo than on either of the in vitro rearing systems; there was no significant difference in the duration of the first instar. Although fecundity and hatch rates were significantly higher for female lice reared in vivo, similar trends have been observed for other membrane-fed arthropods. Body lice (Pediculus humanus L.) and bed bugs (Cimex lectularius [ L.]) also completed most of their life cycle on this apparatus. Our automated mass-rearing system has broad applications for maintaining fluid-sucking ectoparasites and will facilitate various toxicological, behavioral, and disease-transmission investigations.
机译：开发了一种自动饲喂设备以在体外维持人头虱（Pediculus capitis DeGeer）。通过使用阀门和计时器，每隔7天就会将来自冷藏库的储存的人体血液和盐水抽入系统并从系统中冲出。在该旋转间隔期间，通过拉伸的Nescofilm-硅树脂夹心膜连续不断地向头虱提供血液和随意采食。与我们以前的体外人类头部虱子饲养设备相比，可以在最少的人工监视的情况下同时喂入更多的虱子。当体内饲养虱子时，第二至第三龄和第三龄成虫的发育明显快于任一体外饲养系统。第一龄期的持续时间没有显着差异。尽管体内繁殖的雌性虱子的繁殖力和孵化率显着更高，但其他膜饲节肢动物也观察到了相似的趋势。体虱（Pediculus humanus L.）和臭虫（Cimex lectularius [L.]）也在该装置上完成了大部分生命周期。我们的自动批量饲养系统具有广泛的用途，可用于维持吸液性体外寄生虫，并将促进各种毒理学，行为和疾病传播研究。

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