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首页> 外文期刊>Journal of Medical Entomology >Molecular identification of forensically important blow fly species (Diptera : Calliphoridae) in Taiwan
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Molecular identification of forensically important blow fly species (Diptera : Calliphoridae) in Taiwan

机译：台湾法医重要的蝇蝇类（双翅目：Calliphoridae）的分子鉴定

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Forensic entomology is a discipline that mainly uses insects collected in and around corpses to estimate the post-mortem interval in medicocriminal investigations. Among all scavenger and necrophagous insect groups that are related to corpses, blow flies (Diptera: Calliphoridae) are probably most important, not only because they occur in abundant numbers but also because they are one of the earliest groups to find corpses. However, most entomological evidence is strongly dependent on accurate species identification. Because identification allows the proper developmental data and distribution ranges to be applied in criminal investigations, species in Taiwan were surveyed from early 2000 and were identified using molecular data. Currently, eight species have been identified: Chrysomya megacephala (Fabricius), Chrysomya pinguis (Walker), Chrysomya rufifacies (Macquart), Hemipyrellia ligurriens (Wiedemann), Lucilia bazini Seguy, Lucilia cuprina (Wiedemann), Lucilia hainanemis Fan, and Lucilia prophyrina (Walker). We focused on classifying these blow fly species to establish a knowledge basis for further forensic entomological research in Taiwan. Because molecular data are helpful in identifying insect specimens, especially when no specimen of suitable condition for morphological identification is obtained, we extracted mitochondrial cytochrome oxidase subunit I (COI) DNA of the preceding blow fly species to study its application value for their differentiation. The cloning and sequencing of the COI gene (approximate to1,588 base pairs) of these eight species were completed, and the data were analyzed. Preliminary results revealed the high support of congeneric groupings of species by using COI data; these sequences were also shown to be highly conserved within the same species. To actually use the database of COI sequences under various specimen conditions, specific primers were also applied for different insect stages, different segments of adults, and specimens preserved for various times. A molecular primer key was ultimately constructed for the purpose of rapid and accurate species identification at the molecular level regardless of which stage or which part of a blow fly specimen is collected.
机译：法医昆虫学是一门主要使用尸体内部和周围收集的昆虫来估计药物犯罪调查中死后间隔的学科。在所有与尸体相关的清道夫和食尸昆虫组中，蝇蝇（双翅目：Calliphoridae）可能是最重要的，这不仅是因为它们数量众多，而且是发现尸体的最早群体之一。但是，大多数昆虫学证据强烈依赖于准确的物种识别。由于鉴定可以将适当的发展数据和分布范围应用于刑事侦查，因此从2000年初开始对台湾的物种进行调查，并使用分子数据对其进行鉴定。目前，已鉴定出八种：巨型金边霉（Fabricius），扁金边霉（Walker），红褐金边霉（Macquart），斜纹半翅目（Wiedemann），Lucilia bazini Seguy，Lucilia cuprina（Wiedemann），Lucilia hainanemis（Lucilia proinphys）沃克）。我们专注于对这些蝇蝇类进行分类，以为台湾进一步的法医昆虫学研究奠定知识基础。由于分子数据有助于鉴定昆虫标本，尤其是在没有获得适合形态学鉴定的标本的情况下，我们提取了上述蝇蝇的线粒体细胞色素氧化酶亚基I（COI）DNA，以研究其在昆虫分化中的应用价值。这八个物种的COI基因（约1,588个碱基对）的克隆和测序已完成，并分析了数据。初步结果表明，利用COI数据可以对同类物种进行高度支持。这些序列也被证明在同一物种中是高度保守的。为了实际使用各种标本条件下的COI序列数据库，还针对不同的昆虫阶段，成虫的不同部分以及保存了不同时间的标本应用了特定的引物。最终构建了分子引物钥匙，目的是在分子水平上快速，准确地进行物种鉴定，而与采集蝇蝇标本的哪个阶段或哪个部分无关。

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