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首页> 外文期刊>Journal of Medical Entomology >Detection and characterization of Borrelia bissettii in rodents from the central California coast
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Detection and characterization of Borrelia bissettii in rodents from the central California coast

机译：加州中部海岸啮齿动物中疏螺旋体的检测和鉴定

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This is the first report of Borrelia burgdorferi sensu lato in rodents from San Luis Obispo county, with most isolates obtained from a previously unreported host, Neotoma lepida Thomas. B. burgdorferi sensu lato was identified in seven rodent species, including the California vole, Microtus californicus Peale; dusky-footed woodrat, Neotoma fuscipes Baird; desert woodrat, Neotoma lepida Thomas; brush mouse, Peromyscus boylii Baird; California mouse, Peromyscus californicus Gambel; deer mouse, Peromyscus maniculatus Wagner; and western harvest mouse, Reithrodontomys megalotis Baird. Ear punch biopsies were cultured in BSK-H medium from 179 rodents trapped at six different study sites. Overall, prevalence of rodent infection was 44/179 (24.6%), with 34 of these isolates from N. lepida. Spirochete isolates were obtained from rodents at all study sites, indicating widespread prevalence of B. burgdorferi sensu lato across rodent species and habitats. Nucleotide sequences for 14 of these isolates have been submitted to GenBank. Isolates from three N. lepida and one P. boylii had identical flagellin gene sequences, and phylogenetic analysis placed these spirochetes in B. burgdorferi sensu lato group DN127, now known as B. bissettii Postic, Marti Ras, Lane, Hendson & Baranton. Additional sequencing of the intergenic spacer regions between the 5S and 23S ribosomal genes was performed on three of these isolates. Phylogenetic analysis separated these isolates into two clusters that grouped with Colorado or California isolates. The role of B. bissettii and related species other than B. burgdorferi sensu stricto Johnson, Schmid, Hyde, Steigerwalt & Brenner as human pathogens in the United States warrants further investigation.
机译：这是来自圣路易斯·奥比斯波县的啮齿动物中伯氏疏螺旋体的首次报道，大多数分离物是从以前未报道的寄主，Neotoma lepida Thomas获得的。在七个啮齿类动物中鉴定到了伯氏疏螺旋体，其中包括加利福尼亚田鼠，加州田鼠（Microtus californicus Peale）;昏昏欲睡的伍德拉特，Neutoma fuscipes Baird;沙漠伍德拉特（Neotatom lepida Thomas）;刷鼠，Peromyscus boylii Baird;加利福尼亚小鼠Peromyscus californicus Gambel;鹿鼠，Peromyscus maniculatus Wagner;和西部收获的老鼠，大型齿白齿虎（Reithrodontomys megalotis Baird）。在来自六个不同研究地点的179只啮齿动物的BSK-H培养基中培养了耳穿孔活检标本。总体而言，啮齿动物感染的患病率为44/179（24.6％），其中34种来自轻型猪笼草。在所有研究地点均从啮齿类动物中获得了螺旋藻分离株，这表明B. burgdorferi sensu lato在鼠类物种和生境中普遍流行。这些分离物中有14种的核苷酸序列已提交GenBank。来自3个轻链猪笼草和1个博伊氏疟原虫的分离株具有相同的鞭毛蛋白基因序列，并且系统发育分析将这些螺旋体置于B. burgdorferi sensu lato DN127组中，现在称为B. bissettii Postic，Marti Ras，Lane，Hendson＆Baranton。在这些分离物中的三个上，对5S和23S核糖体基因之间的基因间隔区进行了额外的测序。系统发育分析将这些分离株分为两个簇，与科罗拉多或加利福尼亚分离株分组。在美国，B。bissettii和B. burgdorferi sensu stricto以外的其他相关物种作为人类病原体的约翰逊，施密德，海德，施泰格沃特和布伦纳的作用值得进一步研究。

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