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首页> 外文期刊>Journal of Medical Entomology >Host blood meal identification by multiplex polymerase chain reaction for dispersal evidence of stable flies (Diptera: Muscidae) between livestock facilities.
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Host blood meal identification by multiplex polymerase chain reaction for dispersal evidence of stable flies (Diptera: Muscidae) between livestock facilities.

机译：通过多重聚合酶链反应鉴定宿主血粉，以证明家畜设施之间有稳定的苍蝇（双翅目：蝇科）。

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摘要

A species-specific multiplex polymerase chain reaction targeting the cytochrome b gene of cattle, horses, humans, and dogs was developed to determine the blood meal sources of stable flies, Stomoxys calcitrans (L.), collected from Florida equine facilities. Of 595 presumptive blood-fed stable flies analyzed, successful host amplification was obtained in 350, for a field host-detection efficiency of 58.8%. The majority of analyzed stable flies had fed on cattle (64.6%), followed by horses (24.3%), humans (9.5%), and dogs (1.6%). A survey of animal-enclosed pastures occurring within 3 km of stable fly collection sites revealed that the nearest cattle were between 0.8 and 1.5 km from the four horse farm sampling sites. Cattle-feeding frequencies were greater on farms where cattle were located at distances of 0.8 km, suggesting that between farm differences in host-feeding frequency is related to the number of and distance from a particular host type. Time course evaluations of previously laboratory-fed stable flies demonstrated that host-detection efficiency with this system was 100, 50, and 0% when flies were evaluated at 16, 24, and 48 h postblood feeding, respectively. The results of this study suggest short-term stable fly dispersal of up to 1.5 km in a 48-h time period. The implications of these findings are discussed.
机译：开发了针对牛，马，人和狗的细胞色素 b 基因的物种特异性多重聚合酶链反应，以确定稳定果蝇Stomoxys calcitrans（L.）的血粉来源。佛罗里达马设施。在分析的595种假定的食血稳定蝇中，在350种中成功获得了宿主扩增，现场宿主检测效率为58.8％。分析的大多数稳定蝇都以牛为食（64.6％），其次是马（24.3％），人（9.5％）和狗（1.6％）。对在稳定的苍蝇采集点3公里范围内发生的动物围栏牧场进行的一项调查显示，最近的牛距四个马场采样点0.8至1.5公里。在牛距为0.8 km的农场中，牛的饲喂频率更高，这表明农场之间宿主饲喂频率的差异与特定宿主类型的数量和距离有关。以前实验室喂养的稳定蝇的时程评估表明，当在采血后16、24和48 h分别评估蝇时，使用该系统的宿主检测效率分别为100％，50％和0％。这项研究的结果表明，在48小时的时间内，可达1.5 km的短期稳定蝇传播。讨论了这些发现的含义。

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