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首页> 外文期刊>Journal of Medical Entomology >Immuno-chromatographic wicking assay for the rapid detection of dengue viral antigens in mosquitoes (Diptera: Culicidae).
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Immuno-chromatographic wicking assay for the rapid detection of dengue viral antigens in mosquitoes (Diptera: Culicidae).

机译：免疫色谱芯吸测定法可快速检测蚊子中的登革热病毒抗原（双翅目：Cu科）。

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摘要

There is a threat for dengue virus (DENV) reemergence in many regions of the world, particularly in areas where the DENV vectors, Aedes aegypti (L.) and Aedes albopictus (Skuse), are readily available. However, there are currently no accurate and reliable diagnostic methods to provide critical, real-time information for early detection of DENV within the vector populations to implement appropriate vector control and personal protective measures. In this article, we report the ability of an immuno-chromatographic assay developed by VecTOR Test Systems Inc. to detect DENV in a pool of female Aedes mosquitoes infected with any of the four viral serotypes. The DENV dipstick assay was simple to use, did not require a cold chain, and provided clear results within 30 min. It was highly specific and did not cross-react with samples spiked with West Nile, yellow fever, Japanese encephalitis, Rift Valley fever, chikungunya, Venezuelan equine encephalomyelitis, Ross River, LaCrosse, or Caraparu viruses. The DENV assay can provide real-time critical information on the presence of DENV in mosquitoes to public health personnel. Results from this assay will allow a rapid threat assessment and the focusing of vector control measures in high-risk areas.
机译：在世界许多地区，特别是在容易获得登革热媒介，埃及伊蚊和白纹伊蚊的地区，都有登革热病毒再次出现的威胁。但是，目前尚没有准确可靠的诊断方法来提供关键的实时信息，以早期发现病媒中的DENV，以实施适当的病媒控制和个人防护措施。在本文中，我们报告了由VecTOR Test Systems Inc.开发的免疫色谱分析技术能够检测出感染了四种病毒血清型的雌性伊蚊的集合中的DENV的能力。 DENV量油尺测定法易于使用，不需要冷链，并在30分钟内提供了清晰的结果。它具有很高的特异性，并且与加有西尼罗河，黄热病，日本脑炎，裂谷热，基孔肯雅热，委内瑞拉马脑脊髓炎，罗斯河，拉克罗斯或卡拉帕鲁病毒的样品没有交叉反应。 DENV分析可向公共卫生人员提供有关蚊子中DENV的存在的实时关键信息。该分析的结果将允许快速评估威胁，并将重点放在高风险地区的媒介控制措施上。

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