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首页> 外文期刊>Journal of Medical Entomology >Immuno-Chromatographic Wicking Assay for the Rapid Detection of Chikungunya Viral Antigens in Mosquitoes (Diptera: Culicidae)
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Immuno-Chromatographic Wicking Assay for the Rapid Detection of Chikungunya Viral Antigens in Mosquitoes (Diptera: Culicidae)

机译:快速检测蚊子中基孔肯雅病毒抗原的免疫色谱芯吸测定法(双翅目:葫芦科)

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摘要

The outbreak of disease caused by chikungunya virus (CHIKV) in 2006 and the recent spread of this virus to the Americas in 2013 indicate the potential for this virus to spread and cause significant disease. However, there are currently no accurate and reliable field-usable, diagnostic methods to provide critical, real-time information for early detection of CHIKV within the vector populations in order to implement appropriate vector control and personal protective measures. In this article, we report the ability of an immuno-chromatographic assay developed by VecTOR Test Systems Inc. to detect CHIKV in a pool of female Aedes mosquitoes containing a single CHIKV-infected mosquito. The CHIKV dipstick assay was simple to use, did not require a cold chain, and provided clear results within 1 h. It was highly specific and did not cross-react with samples spiked with a variety of other alpha, bunya, and flaviviruses. The CHIKV assay can provide real-time critical information on the presence of CHIKV in mosquitoes to public health personnel. Results from this assay will allow a rapid threat assessment and the focusing of vector control measures in high-risk areas.
机译:2006年由基孔肯雅病毒(CHIKV)引起的疾病暴发以及该病毒在2013年向美洲的最近传播表明该病毒有可能传播并引起重大疾病。但是,目前尚没有准确可靠的现场可用诊断方法来提供关键的实时信息,以及早检测出媒介种群中的CHIKV,从而实施适当的媒介控制和个人防护措施。在本文中,我们报告了由VecTOR Test Systems Inc.开发的免疫色谱测定法检测包含单个CHIKV感染蚊子的雌性伊蚊的池中检测到CHIKV的能力。 CHIKV量油尺测定法易于使用,不需要冷链,并在1小时内提供了清晰的结果。它具有很高的特异性,并且不会与掺有多种其他α,布尼亚病毒和黄病毒的样品发生交叉反应。 CHIKV分析可向公共卫生人员提供有关蚊子中CHIKV存在的实时关键信息。该分析的结果将允许快速评估威胁,并将重点放在高风险地区的媒介控制措施上。

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