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首页> 外文期刊>Journal of Medical Virology >Detection of influenza virus RNA by reverse transcription-PCR and proinflammatory cytokines in influenza-virus-associated encephalopathy.
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Detection of influenza virus RNA by reverse transcription-PCR and proinflammatory cytokines in influenza-virus-associated encephalopathy.

机译：通过逆转录PCR和流感病毒相关脑病中的促炎细胞因子检测流感病毒RNA。

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摘要

Eleven children with acute encephalopathy associated with an influenza virus infection were treated during the 1997-1998 influenza season. Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect the viral genome in peripheral blood and cerebrospinal fluid (CSF) samples. The results were compared with those of control influenza patients without neurological complications. Viral RNA was detected only in the peripheral blood mononuclear cells of one patient with influenza-virus-associated encephalopathy (1 of 9; 11%) and in the CSF of another patient (1 of 11;9%). RT-PCR was negative in the blood of all the controls, but the percentage of RT-PCR-positive samples in the two groups was not significantly different. Cytokines and soluble cytokine receptors in plasma and CSF were then quantified using an enzyme-linked immunosorbent assay. The CSF concentrations of soluble tumor necrosis factor receptor-1 were elevated in two patients and interleukin-6 (IL-6) was elevated in one patient with influenza-virus-associated encephalopathy. On the other hand, the plasma concentrations of IL-6 were elevated in four of nine patients. The number of encephalopathy patients who had elevated plasma concentrations of IL-6 100 pg/ml was significantly higher than that of controls (P= .01). In conclusion, the infrequent detection of the viral genome in the CSF and blood showed that direct invasion of the virus into the central nervous system was an uncommon event. Proinflammatory cytokines and soluble cytokine receptors may mediate the disease. The high plasma concentration of IL-6 could be an indicator of the progression to encephalopathy.
机译：在1997-1998年流感季节，治疗了11例与流感病毒感染有关的急性脑病儿童。逆转录-聚合酶链反应（RT-PCR）分析用于检测外周血和脑脊液（CSF）样品中的病毒基因组。将结果与无神经系统并发症的对照组流感患者进行比较。仅在一名患有流感病毒相关性脑病的患者的外周血单核细胞中检出了病毒RNA（9分之一; 11％），而在另一名患者的CSF中检出了病毒RNA（11分之一; 9％）。所有对照组的血液中RT-PCR均为阴性，但两组中RT-PCR阳性样品的百分比无显着差异。然后使用酶联免疫吸附法对血浆和脑脊液中的细胞因子和可溶性细胞因子受体进行定量。两名患有流感病毒相关性脑病的患者中，可溶性肿瘤坏死因子受体-1的脑脊液浓度升高，白细胞介素6（IL-6）升高。另一方面，九位患者中有四位的血浆IL-6浓度升高。血浆IL-6 100 pg / ml升高的脑病患者人数显着高于对照组（P = .01）。总之，很少在脑脊液和血液中检测到病毒基因组，这表明该病毒直接侵入中枢神经系统是罕见的事件。促炎细胞因子和可溶性细胞因子受体可能介导该疾病。 IL-6的高血浆浓度可能是发展为脑病的指标。

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