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首页> 外文期刊>Journal of Medical Virology >Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens
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Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens

机译：使用常规临床呼吸道标本对新型自动核酸提取系统进行前瞻性评估

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The aim of the study was to evaluate the MagNA Pure 96? nucleic acid extraction system using clinical respiratory specimens for identifying viruses by qualitative real-time PCR assays. Three extraction methods were tested, that is, the MagNA Pure LC?, the COBAS Ampliprep?, and the MagNA Pure 96? with 10-fold dilutions of an influenza A(H1N1)pdm09 sample. Two hundred thirty-nine respiratory specimens, 35 throat swabs, 164 nasopharyngeal specimens, and 40 broncho-alveolar fluids, were extracted with the MagNA Pure 96? and the COBAS Ampliprep? instruments. Forty COBAS Ampliprep? positive samples were also tested. Real-time PCRs were used to identify influenza A and influenza A(H1N1)pdm09, rhinovirus, enterovirus, adenovirus, varicella zoster virus, cytomegalovirus, and herpes simplex virus. Similar results were obtained on RNA extracted from dilutions of influenza A(H1N1)pdm09 with the three systems: the MagNA Pure LC?, the COBAS Ampliprep?, and the MagNA Pure 96?. Data from clinical respiratory specimens extracted with the MagNA Pure 96? and COBAS Ampliprep? instruments were in 98.5% in agreement (P<0.0001) for influenza A and influenza A(H1N1)pdm09. Data for rhinovirus were in 97.3% agreement (P<0.0001) and in 96.8% agreement for enterovirus. They were in 100% agreement for adenovirus. Data for cytomegalovirus and HSV1-2 were in 95.2% agreement (P<0.0001). The MagNA Pure 96? instrument is easy-to-use, reliable, and has a high throughput for extracting total nucleic acid from respiratory specimens. These extracts are suitable for molecular diagnosis with any type of real-time PCR assay.
机译：该研究的目的是评估MagNA Pure 96？核酸提取系统，使用临床呼吸道标本通过定性实时PCR分析鉴定病毒。测试了三种提取方法，即MagNA Pure LC？，COBAS Ampliprep？和MagNA Pure 96?。用10倍稀释的A型流感（H1N1）pdm09样品稀释。用MagNA Pure 96？提取了239个呼吸道标本，35个咽拭子，164个鼻咽标本和40个支气管肺泡液。和COBAS Ampliprep？仪器。四十个COBAS Ampliprep？阳性样品也进行了测试。实时PCR用于鉴定甲型流感和甲型流感（H1N1）pdm09，鼻病毒，肠病毒，腺病毒，水痘带状疱疹病毒，巨细胞病毒和单纯疱疹病毒。用三种系统从甲型流感病毒（H1N1）pdm09的稀释液中提取的RNA获得了相似的结果：MagNA Pure LC？，COBAS Ampliprep？和MagNA Pure 96?。使用MagNA Pure 96？提取的临床呼吸道样本的数据。和COBAS Ampliprep？甲型流感和甲型流感（H1N1）pdm09的检测率达到98.5％（P <0.0001）。鼻病毒的数据一致率为97.3％（P <0.0001），肠病毒的一致率为96.8％。他们同意使用100％的腺病毒。巨细胞病毒和HSV1-2的数据一致率为95.2％（P <0.0001）。 MagNA Pure 96？该仪器易于使用，可靠，并且具有从呼吸道标本中提取总核酸的高通量。这些提取物适用于使用任何类型的实时PCR分析进行分子诊断。

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