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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Cholesterol reduces membrane electroporation and electric deformation of small bilayer vesicles
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Cholesterol reduces membrane electroporation and electric deformation of small bilayer vesicles

机译：胆固醇可减少小双层囊泡的膜电穿孔和电变形

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摘要

Electric fields, similar in the order of magnitude of the natural membrane fields of cellular lipid/protein membranes, and chemical relaxation spectrometry can be used as tools to quantify the rigidifying effect of cholesterol in membranes. Small unilamellar vesicles of radius a = 50 +/- 3 nm, prepared form phosphatidylcholine, phosphatidylserine and phosphatidyl-glycerol in the molar ratio 1:1:1 and containing the optical lipid probe molecule 2-(3-diphenyl-hexatrienyl) propanoyl)-l-paimitoyl-sn-glycerol-3-phosphocholine (beta-DPH pPC), serve as examples for curved lipid membranes. The data of electrooptical turbidity and absorbance relaxations at the wavelength lambda = 365 nm are analysed in terms of membrane bending rigidity K and membrane stretching modulus K. Both kappa and K increase with increasing mole fraction x of cholesterol up to x = 0.5. The cholesterol induced denser packing of the lipids reduces the extent of both membrane electroporation (ME) and electroelongation of the vesicles. Further on, cholesterol in the lipid phase and sucrose in the aqueous suspension reduce the extent of membrane undulation and electro-stretching. (c) 2005 Elsevier B.V All rights reserved.
机译：可以使用类似于细胞脂质/蛋白膜天然膜场数量级的电场和化学弛豫光谱法来量化膜中胆固醇的硬化作用。半径为a = 50 +/- 3 nm的单层小囊泡，由磷脂酰胆碱，磷脂酰丝氨酸和磷脂酰甘油制成，摩尔比为1：1：1，并包含光学脂质探针分子2-（3-二苯基-六三烯基）丙酰基-1-戊二酰基-sn-甘油-3-磷酸胆碱（β-DPHpPC）用作弯曲脂质膜的实例。根据膜的弯曲刚度K和膜的拉伸模量K，分析了在λ＝ 365nm的波长下的电光浊度和吸收率弛豫的数据。κ和K都随着胆固醇的摩尔分数x的增加而增加，直至x ＝ 0.5。胆固醇诱导的脂质的密集堆积降低了膜电穿孔（ME）和囊泡电伸长的程度。此外，脂质相中的胆固醇和水性悬浮液中的蔗糖减少了膜起伏和电拉伸的程度。 （c）2005 Elsevier B.V保留所有权利。

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