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首页> 外文期刊>Journal of molecular microbiology and biotechnology: JMMB >Use of Mycobacterium austroafricanum IFP 2012 in a MTBE-degrading bioreactor
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Use of Mycobacterium austroafricanum IFP 2012 in a MTBE-degrading bioreactor

机译：奥氏分枝杆菌IFP 2012在MTBE降解生物反应器中的用途

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Mycobacterium austroafricanum IFP 2012 is able to slowly grow on methyl tert-butyl ether (MTBE), a fuel oxygenate widely used as a gasoline additive. The potential of M. austroafricanum IFP 2012 for aerobic MTBE degradation was investigated in the presence of a secondary carbon source, isopropanol. The strain was then tested for MTBE biodegradation at the laboratory-scale in a fixed-bed reactor using perlite as the matrix, and isopropanol was injected once a week to maintain M. austroafricanum IFP 2012 biomass inside the perlite bed. The biofilter was operated for 85 days at an influent flow rate of 20 ml/h by varying the MTBE concentration from 10 to 20 mg/l. The hydraulic retention time was fixed at 5 days. The removal of MTBE depended on the inlet MTBE concentration and a MTBE removal efficiency higher than 99% was obtained for MTBE concentrations up to 15 mg/l. A set of 16S rRNA gene primers specific for M. austroafricanum species was used to analyze the DNA extracted from the biofilter effluent in order to detect the presence of M. austroafricanum IFP 2012 and to estimate the effect of periodic injections of isopropanol on the release of the strain from the perlite bed. The results demonstrated that the injection of isopropanol served to maintain an active MTBE degrading biomass in the biofilter and that this system could be used to effectively treat MTBE contaminated groundwater.
机译：南非分枝杆菌IFP 2012能够在甲基叔丁基醚（MTBE）上缓慢生长，MTBE是一种被广泛用作汽油添加剂的含氧燃料。在次要碳源（异丙醇）存在下，研究了奥氏杆菌IFP 2012对好氧MTBE降解的潜力。然后使用珍珠岩作为基质，在固定床反应器中实验室规模测试该菌株的MTBE生物降解性，每周注入异丙醇一次，以在珍珠岩床中保持A.troafricanum IFP 2012生物量。通过将MTBE浓度从10更改为20 mg / l，将生物滤池以20 ml / h的进水流速运行85天。水力停留时间固定为5天。 MTBE的去除取决于进口的MTBE浓度，对于浓度高达15 mg / l的MTBE，其MTBE去除效率高于99％。为了检测从生物滤池出水中提取的DNA，使用了一组特定于奥氏非洲支原体物种的16S rRNA基因引物，以检测奥氏非洲支原体IFP 2012的存在并评估定期注射异丙醇对释放的影响。珍珠岩床中的应变。结果表明，异丙醇的注入可在生物滤池中维持活性MTBE降解活性，并且该系统可用于有效处理受MTBE污染的地下水。

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