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Structural studies of the tRNA domain of tmRNA

机译：tmRNA的tRNA结构域的结构研究

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摘要

tmRNA is a small, stable prokaryotic RNA. It rescues ribosomes that have become stalled during the translation of mRNA fragments lacking stop codons, or during periods of tRNA scarcity. It derives its name from the presence of two separate domains, one that functions as a tRNA, and another that serves as an mRNA. We have carried out modeling and transient electric birefringence studies to determine the angle between the acceptor stem and anticodon stem of the tRNA domain of Eschericia coli tmRNA. The results of the modeling studies yielded an interstem angle of 110 degrees, in agreement with the lower end of the range of angles (111 degrees -137 degrees) determined experimentally for various solution conditions. The range of experimental angles is greater than the angles observed for any of the tRNA crystal structures, in line with the presence of a shortened D stem. The secondary structure of the tRNA domain is conserved for all known tmRNA sequences, so we propose that the angle is also conserved. These results also suggest that the region of tmRNA between P2a and P2b may interact with the decoding site of the ribosome. (C) 2001 Academic Press. [References: 28]
机译：tmRNA是小的，稳定的原核RNA。它可以挽救在缺少终止密码子的mRNA片段翻译过程中或在tRNA缺乏期间已经停滞的核糖体。它的名字源于两个独立域的存在，一个域起着tRNA的作用，另一个域起着mRNA的作用。我们已经进行了建模和瞬态电双折射研究，以确定大肠杆菌tmRNA的tRNA域的受体茎和反密码子茎之间的角度。建模研究的结果产生了110度的中间角，这与针对各种解条件通过实验确定的角度范围的下限（111度-137度）一致。实验角度的范围大于对任何tRNA晶体结构观察到的角度，这与D茎缩短的情况一致。对于所有已知的tmRNA序列，tRNA域的二级结构均是保守的，因此我们建议角度也应保守。这些结果还表明，P2a和P2b之间的tmRNA区域可能与核糖体的解码位点相互作用。 （C）2001学术出版社。 [参考：28]

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