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首页> 外文期刊>Journal of Molecular Biology >The relative position of RyR feet and DHPR tetrads in skeletal muscle
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The relative position of RyR feet and DHPR tetrads in skeletal muscle

机译：RyR脚和DHPR四肢在骨骼肌中的相对位置

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In skeletal muscle, L-type calcium channels (or dihydropyridine receptors, DHPRs) are coupled functionally to the calcium release channels of the sarcoplasmic reticulum (or ryanodine receptors, RyRs) within specialized structures called calcium release units (CRUs). The functional linkage requires a specific positioning of four DHPRs in correspondence of the four identical subunits of a single RyR type 1. Four DHPRs linked to the four binding sites of the RyR1 cytoplasmic domain (or foot), define the corners of a square, constituting a tetrad. RyRs self-assemble into ordered arrays and by associating with them, DHPRs also assemble into ordered arrays. The approximate location of the four DHPRs relative to the four identical subunits of a RyR-foot can be predicted on the basis of the relative position of tetrads and feet within the arrays. However, until recently one vital piece of information has been lacking: the orientation of the two arrays relative to one another. In this work we have defined the relative orientation of the RyR and DHPR arrays by directly superimposing replicas of rotary shadowed images of rows of feet, obtained from isolated SR vesicles, and replicas of tetrad arrays obtained by freeze-fracture. If the orientation for the two sets of images is carefully maintained, the superimposition provides specific constraints on the DHPR-RyR relative position. (C) 2004 Elsevier Ltd. All rights reserved.
机译：在骨骼肌中，L型钙通道（或二氢吡啶受体，DHPRs）在称为钙释放单元（CRUs）的特殊结构内，与肌浆网（或瑞丹碱受体，RyRs）的钙释放通道功能性耦合。功能连接需要四个DHPR的特定定位，以与单个RyR类型1的四个相同亚基相对应。四个DHPR与RyR1细胞质结构域（或脚）的四个结合位点相连，定义了正方形的角，一个四元组。 RyR自组装成有序数组，通过与它们关联，DHPR也组装成有序数组。相对于RyR-脚的四个相同亚基的四个DHPR的大概位置可以根据阵列中四叉和脚的相对位置来预测。但是，直到最近，一个重要的信息仍然缺乏：两个阵列相对于彼此的方向。在这项工作中，我们通过直接叠加从孤立的SR囊泡获得的足行旋转阴影图像的复制品和通过冻裂获得的四元阵列的复制品，来定义RyR和DHPR阵列的相对方向。如果仔细保持两组图像的方向，则叠加将对DHPR-RyR相对位置提供特定的约束。 （C）2004 Elsevier Ltd.保留所有权利。

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