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首页> 外文期刊>Journal of Molecular Biology >Modular structure in developmentally eliminated DNA in Tetrahymena may be a consequence of frequent insertions and deletions.
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Modular structure in developmentally eliminated DNA in Tetrahymena may be a consequence of frequent insertions and deletions.

机译：四膜虫中发育消除的DNA中的模块结构可能是频繁插入和缺失的结果。

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The work reported here describes insertion-deletion (Indel) polymorphisms in two internally eliminated sequences (IESs, that are deleted during development in Tetrahymena): a 1.8 kb Indel at one end of the 1.1 kb H1 IES and a 0.5 kb Indel inside the 1.4 kb calmodulin (C) IES. These two IESs are located in the proximity of the H1 histone and calmodulin genes, respectively, and are among the ten IESs that have been fully sequenced out of an estimated total of 6000. Three hundred base-pairs of the 1.8 kb H1 Indel are retained in the macronucleus. Both the +Indel and the -Indel variants of the H1 and C IESs that occur in different strains are eliminated during development. Thus, a drastic change involving over half of the deleted sequence and 300 bp of flanking sequence does not disable developmental elimination of the H1 IES, which may indicate a lack of requirement for specific sequences on the Indel side of the IES. The H1 Indel is a composite of three sequence elements: a unique segment and two other sections containing members of different repeat families. One of these, a 0.5 kb repetitive component, is 75% similar to another 0.5 kb sequence that constitutes the C Indel, a sequence present in the middle of the calmodulin IES in some strains, but not in others. Therefore, the C Indel sequence is likely to have been part of a mobile unit, even though it has no obvious features of a transposon. However, sequences similar to the C Indel are present in about 100 copies in the genome. The results suggest that IESs may consist, at least in part, of relatively short modules of repeated sequences that are the source of insertion-deletion polymorphisms among strains of Tetrahymena thermophila.
机译：此处报道的工作描述了两个内部消除的序列（IES，在四膜虫的发育过程中被删除）中的插入缺失（Indel）多态性：在1.1 kb H1 IES的一端是一个1.8 kb的Indel，而在1.4内是一个0.5 kb的Indel。 kb钙调蛋白（C）IES。这两个IES分别位于H1组蛋白和钙调蛋白基因的附近，并且是在估计总数为6000个中已完全测序的10个IES中的一个。保留了300个碱基对的1.8 kb H1 Indel。在大核中。 H1和C IES的+ Indel和-Indel变体在不同菌株中均会被消除。因此，涉及超过一半的缺失序列和300bp侧翼序列的剧烈变化不会使H1 IES的发育消除失效，这可能表明在IES的Indel侧上不需要特定序列。 H1 Indel由三个序列元素组成：一个独特的片段和两个包含不同重复家族成员的片段。其中一个（0.5 kb的重复成分）与构成C Indel的另一个0.5 kb序列（与某些菌株的钙调蛋白IES中间存在的序列）相似，为75％。因此，C Indel序列即使没有转座子的明显特征，也很可能是移动单元的一部分。但是，在基因组中约有100个拷贝存在与C Indel相似的序列。结果表明，IES可能至少部分由相对较短的重复序列模块组成，这些模块是嗜热四膜膜虫菌株之间插入缺失多态性的来源。

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