Native hydrogen bonds in a molten globule: The apoflavodoxin thermalintermediate

Irun MP; Garcia-Mira MM; Sanchez-Ruiz JM; Sancho J

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Native hydrogen bonds in a molten globule: The apoflavodoxin thermalintermediate

机译：熔融小球中的天然氢键：载脂蛋白毒素热中间体

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The structure and energetics of protein-folding intermediates are poorly understood. We have identified, in the thermal unfolding of the apoflavodoxin from Anabaena PCC 7119, an equilibrium intermediate with spectroscopic properties of a molten globule and substantial enthalpy and heat capacity of unfolding. The structure of the intermediate is probed by mutagenesis land phi analysis) of polar residues involved in surface-exposed hydrogen bonds connecting secondary-structure elements in the native protein. All hydrogen bonds analysed are formed in the molten globule intermediate, either with native strength or debilitated. This suggests the overall intermediate's topology and surface tertiary interactions are close to native, and indicates that hydrogen bonding may contribute significantly to shape the conformation and energetics of folding intermediates.

机译：人们对蛋白质折叠中间体的结构和能量学知之甚少。我们已经从鱼腥藻PCC 7119的载脂黄素毒素的热展开中发现了一种平衡中间体，该中间体具有熔融小球的光谱特性，并且具有相当大的焓和展开的热容量。中间体的结构通过诱变陆地化学分析来探测，该极性残基涉及与天然蛋白质中二级结构元素相连的表面暴露氢键。所分析的所有氢键均在熔融小球中间体中形成，或者具有天然强度，或者具有衰弱性。这表明整个中间体的拓扑结构和表面三级相互作用都非常接近于天然，并表明氢键可显着影响折叠中间体的构象和能量。

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来源
《Journal of Molecular Biology》 |2001年第4期|共12页
作者
Irun MP; Garcia-Mira MM; Sanchez-Ruiz JM; Sancho J;
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正文语种 eng
中图分类分子生物学;
关键词
Protein stability; Hydrogen bond; Molten globule; Protein intermediate; Protein stability; Alpha-lactalbumin; Conformational stability; Folding; intermediate; Escherichia-coli; Hydrophobic core; Apomyoglobin; Mutagenesis; Mutations; Binding;

机译：蛋白质稳定性;氢键;熔融小球;蛋白质中间体;蛋白质稳定性;α-乳白蛋白;构象稳定性;折叠;中间体;大肠埃希氏大肠杆菌;疏水核心;胞质红蛋白;诱变;突变;结合;

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专利

1. Native hydrogen bonds in a molten globule: The apoflavodoxin thermalintermediate [J] . Irun MP, Garcia-Mira MM, Sanchez-Ruiz JM, Journal of Molecular Biology . 2001,第4期

机译：熔融小球中的天然氢键：载脂蛋白毒素热中间体
2. Noncooperative Formation Of The Off-pathway Molten Globule During Folding Of The α-β Parallel Protein Apoflavodoxin [J] . Sanne M. Nabuurs, Adrie H. Westphal, Carlo P. M. van Mierlo Journal of the American Chemical Society . 2009,第7期

机译：α-β平行蛋白Apoflavodoxin折叠过程中非道路融化小球的非合作形成
3. Equilibrium phi-analysis of a molten globule: the 1-149 apoflavodoxin fragment. [J] . Lopez Llano J, Campos LA, Bueno M, Journal of Molecular Biology . 2006,第2期

机译：熔融小球的平衡phi分析：1-149脱辅基黄素毒素片段。
4. DYNAMICS CHANGES IN THE MOLTEN GLOBULE-NATIVE FOLDING STEP [C] . J. Michael Rowe, Ronald L. Cappelletti, Linda K. Clutter, National Institute of Standards and Technology Center for Neutron Research Accomplishments and Opportunities . 2001

机译：动态变化熔融球褶折叠步骤
5. Protein folding from molten globules to miniature proteins. [D] . Horng, Jia-Cherng. 2004

机译：蛋白质从熔融小球折叠成微型蛋白质。
6. Molten Globule and Native State Ensemble of Helicobacter pylori Flavodoxin: Can Crowding Osmolytes or Cofactors Stabilize the Native Conformation Relative to the Molten Globule? [O] . N. Cremades, J. Sancho 2008

机译：幽门螺杆菌黄热毒素的熔融小球和原生态集合：拥挤渗透压或辅因子能否稳定相对于熔融小球的原生构象？
7. Molten Globule and Native State Ensemble of Helicobacter pylori Flavodoxin: Can Crowding, Osmolytes or Cofactors Stabilize the Native Conformation Relative to the Molten Globule? [O] . Cremades, N., Sancho, J. 2008

机译：幽门螺杆菌黄热毒素的熔融小球和原生态集合：拥挤，渗透压或辅因子能否稳定相对于熔融小球的原生构象？
8. Calculations of Hydrogen-Bonding Interactions between Ions in Room-Temperature Molten Salts [R] . Dymek, C. J., Stewart, J. J. 1989

机译：室温熔盐中离子间氢键相互作用的计算

Native hydrogen bonds in a molten globule: The apoflavodoxin thermalintermediate

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