Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate.

Guerin DM; Lascombe MB; Costabel M; Souchon H; Lamzin V; Beguin P; Alzari PM

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Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate.

机译：与底物复合的反向糖苷酶的原子（0.94 A）拆分结构。

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摘要

The crystal structure of Clostridium thermocellum endoglucanase CelA in complex with cellopentaose has been determined at 0.94 A resolution. The oligosaccharide occupies six D-glucosyl-binding subsites, three on either side of the scissile glycosidic linkage. The substrate and product of the reaction occupy different positions at the reducing end of the cleft, where an extended array of hydrogen-bonding interactions with water molecules fosters the departure of the leaving group. Severe torsional strain upon the bound substrate forces a distorted boat(2,5) B conformation for the glucosyl residue bound at subsite -1, which facilitates the formation of an oxocarbenium ion intermediate and might favor the breakage of the sugar ring concomitant with catalysis.

机译：热纤梭菌内切葡聚糖酶CelA与纤维戊糖复合的晶体结构已确定为0.94 A分辨率。寡糖占据六个D-葡萄糖基结合亚位点，在易裂糖苷键的任一侧上三个。反应的底物和产物在裂隙的还原端占据不同的位置，与水分子的氢键相互作用的扩展阵列促进了离去基团的离开。在结合的底物上的严重扭转应变迫使结合在亚位点-1上的葡糖残基扭曲的舟（2,5）B构象，这有利于氧羰基离子中间体的形成，并可能促进糖环的断裂，并伴随催化作用。

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《Journal of Molecular Biology》 |2002年第5期|共9页
作者
Guerin DM; Lascombe MB; Costabel M; Souchon H; Lamzin V; Beguin P; Alzari PM;
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正文语种 eng
中图分类分子生物学;
关键词
Cellulase; Clostridium; Oligosaccharides; 纤维素酶; 梭菌属; 寡糖类;

机译：Cellulase;Clostridium;Oligosaccharides;纤维素酶;梭菌属;寡糖类;

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1. Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate. [J] . Guerin DM, Lascombe MB, Costabel M, Journal of Molecular Biology . 2002,第5期

机译：与底物复合的反向糖苷酶的原子（0.94 A）拆分结构。
2. Ultrahigh resolution drug design. II. Atomic resolution structures of human aldose reductase holoenzyme complexed with Fidarestat and Minalrestat: implications for the binding of cyclic imide inhibitors. [J] . El Kabbani O, Darmanin C, Schneider TR, Proteins: Structure, Function, and Genetics . 2004,第4期

机译：超高分辨率药物设计。二。与Fidarestat和Minalrestat复合的人醛糖还原酶全酶的原子分辨结构：对环酰亚胺抑制剂结合的影响。
3. Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting alpha-glycosidase [J] . Journal of Structural Biology . 2016,第3期

机译：酶 - 产品复合物的晶体结构揭示了通过家庭63反相α-糖苷酶催化期间的糖环变形
4. Mutational conversion of an inverting glycosidase into a retaining glycosidase having transglycostlation activity [C] . Ryota Kuroki, Brian W.Matthews The Fifth China-Japn joint symposium on enzyme engineering . 1998

机译：将转化的糖苷酶突变为具有转糖基化活性的保留糖苷酶
5. Three-Dimensional Structures at Atomic Resolution: Electron Tomography. [D] . Chen, Chien-Chun. 2013

机译：原子分辨率的三维结构：电子断层扫描。
6. Atomic resolution structures of resting-state substrate- and product-complexed Cu-nitrite reductase provide insight into catalytic mechanism [O] . Svetlana V. Antonyuk, Richard W. Strange, Gary Sawers, 2005

机译：静止态底物和产物复杂的亚硝酸铜还原酶的原子分辨结构提供了对催化机理的了解
7. Atomic (0.94 Å) resolution structure of an inverting glycosidase in complex with substrate [O] . Diego M.A Guérin, Marie-Bernard Lascombe, Marcelo Costabel, 2002

机译：与基材复合物中反相糖苷酶的原子（0.94Å）分辨率结构
8. Visualization of Drug-Nucleic Acid Interactions at Atomic Resolution V. Structure of Two Aminoacridine/Dinucleoside Monophosphate Crystalline Complexes, Proflavine: 5-Iodocytidylyl(3'-5') Guanosine and Acridine Orange: 5-Iodocytidylyl(3'-5') Guanosine [R] . Reddy, B. S., Seshadri, T. P., Sakore, T. D., 1979

机译：原子分辨率下药物 - 核酸相互作用的可视化。两种氨基吖啶/二核苷单磷酸酯结晶复合物的结构，proflavine：5-碘代胞苷（3'-5'）鸟苷和吖啶橙：5-碘代胞苷（3'-5'）鸟苷

Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate.

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