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首页> 外文期刊>Journal of Molecular Biology >The Carboxy-terminal Domain of the DExDH Protein YxiN is Sufficient to Confer Specificity for 23S rRNA.
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The Carboxy-terminal Domain of the DExDH Protein YxiN is Sufficient to Confer Specificity for 23S rRNA.

机译：DExDH蛋白YxiN的羧基末端结构域足以赋予23S rRNA特异性。

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摘要

DExDH proteins are believed to modulate the structures of RNAs and ribonucleoprotein complexes by disrupting RNA helices and RNA-protein interactions. All DExDH proteins contain a two-domain catalytic core that enables their RNA-dependent ATPase and RNA helicase activities. The catalytic core may be flanked by ancillary domains that are proposed to confer substrate specificity and facilitate the unique functions of individual proteins. The Escherichia coli DExDH protein DbpA and its Bacillus subtilis ortholog YxiN have similar 75aa carboxy-terminal domains, and both proteins are specifically targeted to 23S rRNA. Here we demonstrate that the carboxy-terminal domain of YxiN is sufficient to confer RNA specificity by characterizing a chimera in which this domain is appended to the core domains of E.coli SrmB, a DExDH protein with no apparent substrate specificity. Both the RNA-dependent ATPase and RNA helicase activities of the chimera are specifically activated by 23S rRNA and abolished by sequence changes within hairpin 92, a critical recognition element for YxiN. These data support a model in which the carboxy-terminal domain binds hairpin 92 to target the protein to 23S rRNA.
机译：据信DExDH蛋白可通过破坏RNA螺旋和RNA-蛋白相互作用来调节RNA和核糖核蛋白复合物的结构。所有DExDH蛋白都包含一个两个域的催化核心，可实现其RNA依赖性ATPase和RNA解旋酶的活性。催化核心的侧面可能是辅助结构域，该结构域被提议赋予底物特异性并促进单个蛋白质的独特功能。大肠杆菌DExDH蛋白DbpA及其枯草芽孢杆菌直系同源基因YxiN具有相似的75aa羧基末端结构域，并且这两种蛋白都专门针对23S rRNA。在这里，我们证明YxiN的羧基末端结构域足以通过表征嵌合体来赋予RNA特异性，在该嵌合体中，该结构域被附加到大肠杆菌SrmB的核心结构域中，DExDH蛋白没有明显的底物特异性。嵌合体的RNA依赖性ATPase和RNA解旋酶活性均被23S rRNA特异性激活，并被发夹92（YxiN的关键识别元件）内的序列变化所取消。这些数据支持了一个模型，其中羧基末端结构域结合发夹92将蛋白质靶向23S rRNA。

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