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首页> 外文期刊>Nucleic Acids Research >Cloning and biochemical characterization of Bacillus subtilis YxiN, a DEAD protein specifically activated by 23S rRNA: delineation of a novel sub-family of bacterial DEAD proteins.
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Cloning and biochemical characterization of Bacillus subtilis YxiN, a DEAD protein specifically activated by 23S rRNA: delineation of a novel sub-family of bacterial DEAD proteins.

机译：枯草芽孢杆菌YxiN（一种由23S rRNA特异性激活的DEAD蛋白）的克隆和生化特征：细菌DEAD蛋白的新亚家族的描述。

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摘要

DEAD, DEAH and DExH proteins are involved in almost every facet of RNA biochemistry. Members of these protein families exhibit an RNA-dependent ATPase activity and some possess an ATP-dependent RNA helicase activity. Although genetic studies have identified specific functions for certain DEx(D)/(H)proteins from which an RNA substrate can be reasonably inferred, only DbpA from Escherichia coli has been shown to exhibit significant RNA specificity in vitro. Here we describe the characterization of YxiN from Bacillus subtilis, the second DEx(D)/(H)protein to show significant RNA specificity as an isolated, homogenous protein. The ATPase activity of YxiN, like that of DbpA, is stimulated by a 154 nt fragment of 23S rRNA. YxiN has a 2 nM apparent binding constant for this fragment, yet its ATPase activity shows 1800-fold RNA specificity. Along with the conserved motifs shared among all DEAD proteins, YxiN and DbpA have a conserved C-terminal extension. This extension is highly conserved in several additional DEAD proteins. We propose that the C-terminus identifies a protein sub-family whose members bind 23S rRNA and that proteins of this family are likely to function in rRNA maturation/ribosome biogenesis or an unappreciated aspect of translation.
机译：DEAD，DEAH和DExH蛋白几乎涉及RNA生物化学的各个方面。这些蛋白质家族的成员表现出RNA依赖性的ATPase活性，而某些家族具有ATP依赖性的RNA解旋酶的活性。尽管遗传学研究已经确定了可以从中合理推断出RNA底物的某些DEx（D）/（H）蛋白的特定功能，但是只有来自大肠杆菌的DbpA在体外显示出显着的RNA特异性。在这里，我们描述了来自枯草芽孢杆菌的YxiN的特征，第二个DEx（D）/（H）蛋白显示出显着的RNA特异性，为分离的同质蛋白。与DbpA一样，YxiN的ATPase活性被23S rRNA的154 nt片段刺激。 YxiN对于该片段具有2 nM的表观结合常数，但其ATPase活性显示出1800倍的RNA特异性。除所有DEAD蛋白中共有的保守基序外，YxiN和DbpA还具有保守的C端延伸。这种延伸在其他几种DEAD蛋白中高度保守。我们建议，C末端标识一个蛋白质亚家族，其成员结合23S rRNA，并且该家族的蛋白质很可能在rRNA成熟/核糖体生物发生或翻译的未受重视的方面发挥作用。

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