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首页> 外文期刊>Journal of Molecular Biology >Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy.
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Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy.

机译：在葡萄球菌核酸酶的N末端大片段中揭示出类似天然的部分折叠的构象和折叠过程：通过NMR光谱研究。

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摘要

The N-terminal large fragments of staphylococcal nuclease (SNase), SNase110 (1-110 residues), SNase121 (1-121 residues), and SNase135 (1-135 residues), and the fragment mutants G88W110, G88W121, V66W110 and V66W121 were studied by heteronuclear multidimensional NMR spectroscopy. Ensembles of co-existent native-like partially folded and unfolded states were observed for fragments. The persistent native-like tertiary interaction drives fragments to be in partially folded states, which reveal native-like beta-barrel conformations. G88W and V66W mutations modulate the extent of inherent native-like tertiary interaction in fragment molecules, and in consequence, fragment mutants fold into native-like beta-subdomain conformations. In cooperation with the inherent tertiary interaction, 2 M TMAO (trimethylamine N-oxide) can promote the folding reaction of fragments through the changes of unfolding free energy, and a native-like beta-subdomain conformation is observed when the chain length contains 135 residues. Heterogeneous partially folded conformations of 1-121 and 1-135 fragments due to cis and trans X-prolyl bond of Lys116-Pro117 make a non-unique folding pathway of fragments. The folding reaction of fragments can be characterized as a hierarchical process.
机译：葡萄球菌核酸酶（SNase），SNase110（1-110残基），SNase121（1-121残基）和SNase135（1-135残基）的N末端大片段以及片段突变体G88W110，G88W121，V66W110和V66W121是通过异核多维NMR光谱研究。观察到共存的天然样的部分折叠和未折叠状态的集合的片段。持久的类似自然的三级相互作用驱使片段处于部分折叠状态，这揭示了类似自然的β-桶构象。 G88W和V66W突变调节片段分子中固有的天然样三级相互作用的程度，因此，片段突变体折叠成天然样β亚结构域构象。与固有的三次相互作用配合，2 M TMAO（三甲胺N-氧化物）可以通过展开自由能的变化促进片段的折叠反应，并且当链长包含135个残基时观察到天然的β-亚结构域构象。由于Lys116-Pro117的顺式和反式X-脯氨酰键，1-121和1-135片段的异质部分折叠的构象构成了片段的非唯一折叠途径。片段的折叠反应可以表征为分级过程。

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