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首页> 外文期刊>Journal of Molecular Biology >The sensitivity of lipid domains to small perturbations demonstrated by the effect of triton.
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The sensitivity of lipid domains to small perturbations demonstrated by the effect of triton.

机译：Triton的作用证明了脂质结构域对小扰动的敏感性。

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摘要

The hypothesis of lipid rafts describes functional domains in biological membranes. It is often assumed that rafts form by spontaneous de-mixing of certain lipids and that they can be isolated as detergent-resistant membrane particles (DRMs) using the detergent Triton X-100 (TX). Here, we present a model that describes the process of domain formation in membranes in the presence and in the absence of TX. We measure the interactions between TX and an equimolar mixture of sphingomyelin (SM), cholesterol (Cho), and 1-palmitoyl-2-oleoyl-3-sn-glycero-phosphatidylcholine (POPC) (1:1:1, mol) by means of isothermal titration calorimetry. Comparison with pure POPC membranes reveals a very unfavorable interaction between TX and SM/Cho, which causes a substantial tendency to segregate these molecules into separate, DRM-like (SM-rich) and fluid (TX-rich), domains. If rafts are indeed formed by spontaneous de-mixing of PC and SM/Cho, they must be very sensitive, and perturbations caused by techniques used to study rafts could lead to misleading results. If, however, rafts are much more stable than PC-SM-Cho domains, there must be an unknown raft stabilizer. Subtle changes of such a promoter could serve to modulate raft function.
机译：脂质筏的假说描述了生物膜中的功能域。通常认为筏是通过某些脂质的自发分解而形成的，并且可以使用洗涤剂Triton X-100（TX）将其分离为抗洗涤剂的膜颗粒（DRM）。在这里，我们提出一个模型，描述存在和不存在TX的情况下在膜中形成域的过程。我们通过以下方法测量TX与鞘磷脂（SM），胆固醇（Cho）和1-palmitoyl-2-oleoyl-3-sn-甘油磷脂酰胆碱（POPC）（1：1：1，mol）的等摩尔混合物之间的相互作用等温滴定量热法。与纯POPC膜的比较显示TX和​​SM / Cho之间存在非常不利的相互作用，这导致将这些分子分离为单独的，DRM样（富含SM）和流体（富含TX）域的明显趋势。如果确实通过PC和SM / Cho的自发混合形成了筏，则它们必须非常敏感，并且用于研究筏的技术引起的扰动可能导致误导性的结果。但是，如果筏比PC-SM-Cho域稳定得多，则必须有一个未知的筏稳定器。这种启动子的细微变化可用于调节筏功能。

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