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首页> 外文期刊>Journal of Molecular Biology >Functional analysis of BamHI DNA cytosine-N4 methyltransferase.
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Functional analysis of BamHI DNA cytosine-N4 methyltransferase.

机译：BamHI DNA胞嘧啶-N4甲基转移酶的功能分析。

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摘要

We show that the kinetic mechanism of the DNA (cytosine-N(4)-)-methyltransferase M.BamHI, which modifies the underlined cytosine (GGATCC), differs from cytosine C(5) methyltransferases, and is similar to that observed with adenine N(6) methyltransferases. This suggests that the obligate order of ternary complex assembly and disassembly depends on the type of methylation reaction. In contrast, the single-turnover rate of catalysis for M.BamHI (0.10s(-1)) is closer to the DNA (cytosine-C(5)-)-methyltransferases (0.14s(-1)) than the DNA (adenine-N(6)-)-methyltransferases (>200s(-1)). The nucleotide flipping transition dominates the single-turnover constant for adenine N(6) methyltransferases, and, since the disruption of the guanine-cytosine base-pair is essential for both types of cytosine DNA methyltransferases, this transition may be a common, rate-limiting step for methylation for these two enzyme subclasses. The similar overall rate of catalysis by M.BamHI and other DNA methyltransferases is consistent with a common rate-limiting catalytic step of product dissociation. Our analyses of M.BamHI provide functional insights into the relationship between the three different classes of DNA methyltransferases that complement both prior structural and evolutionary insights.
机译：我们表明，DNA（胞嘧啶-N（4）-）甲基转移酶M.BamHI的动力学机制，修改下划线的胞嘧啶（GGATCC），不同于胞嘧啶C（5）甲基转移酶，并且类似于腺嘌呤N（6）甲基转移酶。这表明三元复合物组装和拆卸的专性顺序取决于甲基化反应的类型。相反，M.BamHI（0.10s（-1））的单周转率比DNA（胞嘧啶-C（5）-）-甲基转移酶（0.14s（-1））更接近DNA（腺嘌呤-N（6）-）-甲基转移酶（> 200s（-1））。核苷酸翻转转变主导着腺嘌呤N（6）甲基转移酶的单周转常数，而且由于鸟嘌呤-胞嘧啶碱基对的破坏对于两种类型的胞嘧啶DNA甲基转移酶都是必不可少的，因此这种转变可能是常见的，这两个酶亚类的甲基化限制步骤。 M.BamHI和其他DNA甲基转移酶的总体催化速率相似，这与产物解离的常见限速催化步骤一致。我们对M.BamHI的分析提供了对三种不同类别的DNA甲基转移酶之间关系的功能性见解，可补充先前的结构和进化见解。

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