• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Dual Role of boxB RNA Motif in the Mechanisms of Termination/Antitermination at the Lambda tR1 Terminator Revealed in vivo.
【24h】

Dual Role of boxB RNA Motif in the Mechanisms of Termination/Antitermination at the Lambda tR1 Terminator Revealed in vivo.

机译：在体内揭示了boxB RNA基序在Lambda tR1终止子的终止/抗终止机制中的双重作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rho-dependent transcription termination at the phage lambda tR1 terminator is governed primarily by the upstream rut element that encodes two RNA regions rutA and rutB. The two regions are separated by the boxB RNA motif, which is believed to be dispensable for Rho activity but serves as a binding site for lambda N protein in the antitermination process. By using a minimal in vivo termination system, we show that the intervening boxB RNA motif has a double function in the mechanisms of termination/antitermination at lambdatR1. As a folded hairpin structure, it acts as a clamp that holds rutA and rutB side by side for optimal interactions with Rho leading to efficient termination. Conversely, the binding of N protein to boxB induces antitermination at lambdatR1 by preventing access of Rho to the rut sequences. This dual role was clearly shown in vivo by studying the effects of multiple mutations within the boxB hairpin stem on transcription termination and by substituting the N/boxB couple with the unrelated coat protein of phage MS2 and its stem-loop RNA binding site.
机译：在噬菌体λtR1终止子处的Rho依赖性转录终止主要由上游的rut元件控制，该元件编码两个RNA区域rutA和rutB。这两个区域被boxB RNA基序分隔开，boxB RNA基序被认为对Rho活性是可有可无的，但在抗终止过程中充当了λN蛋白的结合位点。通过使用最小限度的体内终止系统，我们表明，干预的boxB RNA基序在lambdatR1的终止/抗终止机制中具有双重功能。作为折叠的发夹结构，它可充当并排固定rutA和rutB的夹子，以实现与Rho的最佳相互作用，从而有效终止。相反，N蛋白与boxB的结合通过阻止Rho进入车辙序列而在lambdatR1诱导抗终止作用。通过研究boxB发夹茎内多个突变对转录终止的影响，以及通过用噬菌体MS2的无关外壳蛋白及其茎环RNA结合位点取代N / boxB偶联，在体内清楚显示了这种双重作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号