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首页> 外文期刊>Journal of Molecular Biology >ANALYSIS OF THE LOW-FREQUENCY NORMAL MODES OF THE R STATE OF ASPARTATE TRANSCARBAMYLASE AND A COMPARISON WITH THE T STATE MODES
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ANALYSIS OF THE LOW-FREQUENCY NORMAL MODES OF THE R STATE OF ASPARTATE TRANSCARBAMYLASE AND A COMPARISON WITH THE T STATE MODES

机译：天冬氨酸转氨酶R状态的低频正常模式分析及与T状态模式的比较

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摘要

Aspartate transcarbamylase (ATCase) is a classic example of an allosteric enzyme. It catalyzes the conversion of aspartate to carbamyl aspartate, which is the first substrate in the biosynthesis of pyrimidines. Although ATCase is well characterized, both structurally and biochemically, little is known at the atomic level about the large amplitude motions that govern its T-->R quaternary transition. We present the results of calculations of the very-low-frequency normal modes of the CTP-ligated R state ATCase, and we compare them with the equivalent modes in the CTP-ligated T state ATCase. The large-amplitude, delocalized modes of frequencies below 4 cm(-1) contribute a large fraction of the atomic fluctuations observed experimentally. They show some ability to drive the R-state structure towards the T-state structure, by promoting some of the quaternary structure rearrangements that take place during the allosteric process. Their potential role in the T-->R transition is quantified and compared with the role of the low-frequency modes of the T state in the quaternary rearrangement. (C) 1996 Academic Press Limited. [References: 46]
机译：天冬氨酸转氨甲酰酶（ATCase）是变构酶的经典例子。它催化天冬氨酸向氨基甲酸酯天冬氨酸的转化，这是嘧啶生物合成中的第一个底物。尽管ATCase在结构和生化上都有很好的表征，但在原子水平上对控制其T-> R四元跃迁的大振幅运动知之甚少。我们介绍了CTP连接的R状态ATCase的极低频正常模式的计算结果，并将它们与CTP连接的T状态ATCase的等效模式进行了比较。低于4 cm（-1）的频率的大振幅，离域模式贡献了实验观察到的大部分原子波动。通过促进在变构过程中发生的一些四元结构重排，它们显示出将R态结构朝T态结构驱动的能力。量化了它们在T-> R跃迁中的潜在作用，并将其与T状态的低频模式在四元重排中的作用进行了比较。 （C）1996 Academic Press Limited。 [参考：46]

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