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首页> 外文期刊>Journal of Molecular Biology >POSSIBLE ROLES OF HIV-1 NUCLEOCAPSID PROTEIN IN THE SPECIFICITY OF PROVIRAL DNA SYNTHESIS AND IN ITS VARIABILITY
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POSSIBLE ROLES OF HIV-1 NUCLEOCAPSID PROTEIN IN THE SPECIFICITY OF PROVIRAL DNA SYNTHESIS AND IN ITS VARIABILITY

机译：HIV-1核衣壳蛋白在原DNA合成的特异性及其变异中的可能作用

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Retroviral nucleocapsid (NC) protein is an integral part of the virion nucleocapsid where it coats the dimeric RNA genome, Due to its nucleic acid binding and annealing activities, NC protein directs the annealing of the tRNA primer to the primer binding site and greatly facilitates minus strand DNA elongation and transfer while protecting the nucleic acids against nuclease degradation. To understand Me role of NCp7 In viral DNA synthesis, we examined Me influence of NCp7 on self-primed versus primer-specific reverse transcription. The results show that HIV-1 NCp7 can extensively inhibit sell-primed reverse transcription of viral and cellular RNAs while promoting primer-specific synthesis of proviral DNA. The role of NCp7 vis-a-vis the. presence of mutations in the viral DNA during minus strand elongation was examined, NCp7 maximized the annealing between a cDNA(-) primer containing one to five consecutive errors and an RNA representing the 3' end of the genome. The ability of reverse transcriptase (RT) in the presence of NCp7 to subsequently extend the mutated primers depended upon the position of the mismatch within the primer:template complex. When the mutations were at the polymerisation site, primer extension by RT in the presence of NCp7 was very high, about 40% for one mismatch and 3% for five consecutive mismatches. Mutations within the DNA primer or at its 5' end had Little effect on the extension of viral DNA by RT. Taken together these results indicate that NCp7 plays major roles in proviral DNA synthesis within the virion core due to its ability to promote prime-specific proviral DNA synthesis while concurrently inhibiting non-specific reverse transcription of viral and cellular RNAs. Moreover, the observation that NCp7 enhances the incorporation of mutations during minus strand DNA elongation favours the notion that NCp7 is a factor contributing to the high mutation rate of HIV-1. (C) 1997 Academic Press Limited. [References: 61]
机译：逆转录病毒核衣壳（NC）蛋白是病毒颗粒核衣壳不可或缺的部分，在那里它包裹着二聚体RNA基因组。由于其核酸结合和退火活性，NC蛋白将tRNA引物的退火引导到引物结合位点，极大地促进了减号链DNA的延伸和转移，同时保护核酸免受核酸酶降解。为了了解NCp7在病毒DNA合成中的作用，我们检查了NCp7对自我引物和引物特异性逆转录的影响。结果表明，HIV-1 NCp7可以广泛抑制病毒和细胞RNA的出售引发的逆转录，同时促进前病毒DNA的引物特异性合成。相对于NCp7的作用。检查了负链延伸过程中病毒DNA中突变的存在，NCp7使包含一到五个连续错误的cDNA（-）引物与代表基因组3'端的RNA之间的退火最大化。在存在NCp7的情况下逆转录酶（RT）随后扩展突变引物的能力取决于引物：模板复合物中错配的位置。当突变位于聚合位点时，存在NCp7时RT引物延伸非常高，一个错配约为40％，五个连续错配约为3％。 DNA引物内或其5'端的突变对RT扩增病毒DNA的影响很小。归纳起来，这些结果表明NCp7在病毒体核心内的前病毒DNA合成中起主要作用，因为它能够促进主要特异性前病毒DNA合成，同时抑制病毒和细胞RNA的非特异性逆转录。此外，观察到NCp7增强了负链DNA延伸过程中突变的掺入，这支持了NCp7是导致HIV-1高突变率的因素的观点。 （C）1997 Academic Press Limited。 [参考：61]

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