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首页> 外文期刊>Journal of Molecular Biology >EVIDENCE FOR INVOLVEMENT OF ESCHERICHIA COLI GENES PMBA, CSRA AND A PREVIOUSLY UNRECOGNIZED GENE TLDD, IN THE CONTROL OF DNA GYRASE BY LETD (CCDB) OF SEX FACTOR F
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EVIDENCE FOR INVOLVEMENT OF ESCHERICHIA COLI GENES PMBA, CSRA AND A PREVIOUSLY UNRECOGNIZED GENE TLDD, IN THE CONTROL OF DNA GYRASE BY LETD (CCDB) OF SEX FACTOR F

机译：在性因子F的LETD（CCDB）控制DNA合成酶的过程中，涉及大肠埃希氏菌PMBA，CSRA和以前无法识别的基因TLDD参与的证据

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The letA (ccdA) and letD (ccdB) genes of the F plasmid, located just outside the sequence essential for replication, contribute to stable maintenance of the plasmid in Escherichia coli cells. The letD gene product acts to inhibit partitioning of chromosomal DNA and cell division by inhibiting DNA gyrase activity, whereas the letA gene product acts to reverse the inhibitory activity of the letD gene product. To identify the host factor(s) involved in this process, we analyzed the mutants that escaped letD expression and their suppressor, and found that the three E., coli genes tldD, tldE and zfiA participate in the process, in addition to the groE genes we reported previously The tldD and tldE mutations made cells tolerant for letD expression, as did groES mutations, while the mutation in the zfiA gene made tldD, tldE and groES mutants LetD sensitive. We hypothesize that these gene products are factors that modulate activity of DNA gyrase along with the letD gene product; the zfiA gene product acts to inhibit interaction between the LetD protein and the A subunit of DNA gyrase, while the tldD, tldE and groE gene products act to suppress the inhibitory activity of the zfiA gene product. The tldD, tldE, and zfiA genes are located at 70.4, 96.0 and 58.2 minutes on the E. coli chromosome, respectively, and code for proteins with relative molecular masses of 51,000, 48,000 and 6800, respectively. tldD is a novel gene, but the tldE and zfiA genes proved to be the pmbA gene (production of Microcin B17) and the csrA gene (carbon storage regulator), respectively. (C) 1996 Academic Press Limited [References: 48]
机译：F质粒的letA（ccdA）和letD（ccdB）基因位于复制必不可少的序列之外，它们有助于质粒在大肠杆菌细胞中的稳定维持。 letD基因产物通过抑制DNA促旋酶活性来抑制染色体DNA的分配和细胞分裂，而letA基因产物的作用是逆转letD基因产物的抑制活性。为了确定此过程中涉及的宿主因子，我们分析了逃脱letD表达的突变体及其抑制因子，发现除了groE之外，还有三个大肠杆菌基因tldD，tldE和zfiA参与了该过程。我们先前报道的基因tldD和tldE突变使细胞对letD表达具有耐受性，而groES突变也是如此，而zfiA基因的突变使tldD，tldE和groES突变体对LetD敏感。我们假设这些基因产物是与letD基因产物一起调节DNA促旋酶活性的因子。 zfiA基因产物的作用是抑制LetD蛋白与DNA旋转酶的A亚基之间的相互作用，而tldD，tldE和groE基因产物的作用是抑制zfiA基因产物的抑制活性。 tldD，tldE和zfiA基因分别位于大肠杆菌染色体上的70.4、96.0和58.2分钟，并分别编码相对分子质量为51,000、48,000和6800的蛋白质。 tldD是一个新基因，但是tldE和zfiA基因分别被证明是pmbA基因（Microcin B17的生产）和csrA基因（碳储存调节剂）。 （C）1996 Academic Press Limited [参考号：48]

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