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首页> 外文期刊>Journal of Molecular Biology >SELECTIVE PHAGE INFECTION MEDIATED BY EPITOPE EXPRESSION ON F PILUS
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SELECTIVE PHAGE INFECTION MEDIATED BY EPITOPE EXPRESSION ON F PILUS

机译：表皮表达在F P上介导的选择性噬菌体感染

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摘要

Proteins and peptides can be displayed on bacterial curd bacteriophage surfaces as fusions to bacterial integral membrane proteins or phage coat proteins. We now report on the expression of peptide antigens on the surface of F pill, elaborated by F+ strains of Escherichia toll. The peptides were expressed as fusions to F pilin, the building block of the? F pilus that is encoded by the traA gene on the F plasmid. Filamentous bacteriophage infection of E. coli is normally mediated by phage binding to pilin at the F pill tip. Expression of 13 to 15 amino acid long peptides on the F pilus completely blocked infection by derivatives of wild-type infectious M13 phage. However, when a phage displaying a specific recombinant antibody fragment was allowed to interact with F pili displaying an antigenic peptide a bacterial infection could be demonstrated. This infection, mediated by the antibody-antigen interaction, resulted In bacterial cells containing plasmids encoding both the protein and the ligand, in a model library, where a scFv antibody against the human cytomegalovirus AD-2 epitope was selected we achieved an enrichment of 2500 of phage carrying the specific antibody, indicating an efficient selective Infection. (C) 1997 Academic Press Limited. [References: 25]
机译：蛋白质和多肽可以与细菌完整膜​​蛋白或噬菌体外壳蛋白融合，显示在细菌凝乳噬菌体表面。现在，我们报告由F +株大肠杆菌造成的F丸表面上的多肽抗原表达。所述肽表达为与F pilin（β-结构的组成部分）的融合体。 F菌毛，由F质粒上的traA基因编码。大肠杆菌的丝状噬菌体感染通常由噬菌体与F药丸尖端的菌毛蛋白结合介导。 F菌毛上13至15个氨基酸长的肽表达完全阻断了野生型感染性M13噬菌体衍生物的感染。然而，当将展示特定重组抗体片段的噬菌体与展示抗原肽的F菌毛相互作用时，可以证明细菌感染。通过抗体-抗原相互作用介导的这种感染导致在模型库中包含同时编码蛋白质和配体的质粒的细菌细胞，在该库中选择了针对人巨细胞病毒AD-2表位的scFv抗体，我们获得了2500的富集度携带特异性抗体的噬菌体的数量，表明有效的选择性感染。 （C）1997 Academic Press Limited。 [参考：25]

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