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首页> 外文期刊>Journal of Molecular Biology >CHARACTERIZATION OF A NON-LONG TERMINAL REPEAT RETROTRANSPOSON CDNA (L1TC) FROM TRYPANOSOMA CRUZI - HOMOLOGY OF THE FIRST ORF WITH THE APE FAMILY OF DNA REPAIR ENZYMES
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CHARACTERIZATION OF A NON-LONG TERMINAL REPEAT RETROTRANSPOSON CDNA (L1TC) FROM TRYPANOSOMA CRUZI - HOMOLOGY OF THE FIRST ORF WITH THE APE FAMILY OF DNA REPAIR ENZYMES

机译：克鲁氏锥虫的非长末端重复反转录转座子CDNA（L1TC）的特征-第一个ORF的同源性与DNA修复酶的猿类

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In the present paper we describe the characterization of a Trypanosoma cruzi cDNA (L1Tc) corresponding to a transcript from a new long terminal repeat (LTR) retrotransposon This element is present in a high-copy number, and is found dispersed throughout the T. cruzi genome. Northern analysis shows an abundant expression of L1Tc-related sequences with a major band of about 5 kb. The transcript has at its 3' end a fragment of a highly repetitive DNA sequence (E12A), at its 5' end a ribosomal mobile element-like sequence and three putative open reading frames (ORF) in different frames. The ORF2 codes for a protein which has significant homology with the retrotranscriptase-related sequences from non-LTR retrotransposons containing the seven domains present in all the retrotranscriptase and retrotranscriptase-related proteins. The ORF3 codes for a gag-like protein showing unusual cysteine motifs present in all non-LTR trypanosomatid elements, similar to the C2H2 zinc finger family of transcription factors. Interestingly, ORF1 codes for a protein with significant homology to the major human AP endonuclease protein, and maintains in similar positions most of the amino acid domains described for ah the Ape family of proteins. The presence of Ape-related sequences, described for the first time in a non-LTR retrotransposon (L1Tc), may have functional relevance for these types of elements. [References: 51]
机译：在本文中，我们描述了锥虫锥虫cDNA（L1Tc）的特征，该cDNA对应于来自新的长末端重复序列（LTR）反转录转座子的转录本。该元件以高拷贝数存在，并且分散在整个克氏锥虫中基因组。 Northern分析显示L1Tc相关序列的大量表达，主带约为5 kb。转录本在其3'端具有一个高度重复的DNA序列（E12A）的片段，在其5'端具有一个核糖体移动元件样序列以及三个不同框架中的推定开放阅读框（ORF）。 ORF2编码一种蛋白质，该蛋白质与来自非LTR逆转座子的逆转录酶相关序列具有显着同源性，该非LTR逆转座子包含存在于所有逆转录酶和逆转录酶相关蛋白中的七个结构域。 ORF3编码一种gag样蛋白，显示出在所有非LTR锥虫元素中都存在异常的半胱氨酸基序，类似于C2H2锌指家族的转录因子。有趣的是，ORF1编码与主要人类AP核酸内切酶蛋白具有显着同源性的蛋白，并且将Ape蛋白家族中描述的大多数氨基酸结构域保持在相似的位置。非LTR反转录转座子（L1Tc）中首次描述的Ape相关序列的存在可能与这些类型的元件具有功能相关性。 [参考：51]

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