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首页> 外文期刊>Journal of Molecular Biology >A THERMODYNAMIC STUDY OF MUTANT FORMS OF STREPTOMYCES SUBTILISIN INHIBITOR .1. HYDROPHOBIC REPLACEMENTS AT THE POSITION OF METL03
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A THERMODYNAMIC STUDY OF MUTANT FORMS OF STREPTOMYCES SUBTILISIN INHIBITOR .1. HYDROPHOBIC REPLACEMENTS AT THE POSITION OF METL03

机译：热乳链球菌抑菌酶抑制剂突变形式的热力学研究.. 1。 METL03位置的疏水替换

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摘要

The thermodynamic effects of replacing the Met residue at amino acid position 103 of Streptomyces subtilisin inhibitor with other non-polar aliphatic residues were studied by means of differential scanning calorimetry All but the Leu mutant, which is as stable as the wild-type but has different cooperative units in the course of unfolding, showed destabilization in terms of free energy Similar losses in free energy, however, were caused by different reasons, i.e. by increased entropy for the Ala mutant and by decreased enthalpy for the Ile mutant, with a tendency that increases in entropy are accompanied by increases in enthalpy. The gain in entropy that caused the largest loss in free energy for the Gly mutant was unexpectedly smaller than that for the Ala mutant. The changes in enthalpy and entropy induced by the mutations exhibited some correlations with hydrophobicity, while no clear correlation was found between the changes in free energy and hydrophobicity. [References: 39]
机译：通过差示扫描量热法研究了用其他非极性脂肪族残基取代链霉菌枯草杆菌蛋白酶抑制剂第103位氨基酸的Met残基的热力学效应。合作单元在展开过程中表现出的自由能不稳定，然而，自由能的类似损失是由不同原因引起的，例如，Ala突变体的熵增加和Ile突变体的焓降低，其趋势是熵的增加伴随着焓的增加。导致Gly突变体最大自由能损失的熵增益出乎意料地小于Ala突变体。突变引起的焓和熵的变化与疏水性表现出一定的相关性，而自由能和疏水性的变化之间没有明显的相关性。 [参考：39]

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