• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >ASSEMBLY OF FUNCTIONAL BACTERIOPHAGE LAMBDA VIRIONS INCORPORATING C-TERMINAL PEPTIDE OR PROTEIN FUSIONS WITH THE MAJOR TAIL PROTEIN
【24h】

ASSEMBLY OF FUNCTIONAL BACTERIOPHAGE LAMBDA VIRIONS INCORPORATING C-TERMINAL PEPTIDE OR PROTEIN FUSIONS WITH THE MAJOR TAIL PROTEIN

机译：功能性噬菌体λ病毒与主要尾巴蛋白融合的C端肽或蛋白融合体的组装

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The tolerance of bacteriophage lambda morphogenesis for C-terminal additions to the tail tube major protein subunit (the V gene product; gpV) has been investigated. A second modified copy of the lambda V gene, either within a novel phage vector itself or plasmid-borne, was expressed during phage growth. High-level substitution of wild-type gpV by modified gpV bearing a basic C-terminal peptide sequence (RRASV; a target site for cAMP-dependent protein kinase) was possible using multiple repeats of a serine-glycine (SGGG) linker sequence. Highly purified phage bearing copies of gpV-RRASV could be efficiently phosphorylated by the appropriate protein kinase, and the incorporated label was shown to migrate exclusively at the expected size in protein gels. A large tetrameric protein (beta-galactosidase) could be incorporated into active virions in at least one copy, again using a Ser-Gly linker. These studies suggest that with a suitable spacing linker and controlled levels of expression, it is likely that a wide range of protein or peptide substituents can be fused with gpV at its C terminus and assembled as component sub units of the tail tube. [References: 33]
机译：已经研究了噬菌体λ形态发生对尾管主要蛋白亚基（V基因产物; gpV）C末端添加的影响。在噬菌体生长过程中，表达了λV基因的第二个修饰拷贝，该拷贝是在新型噬菌体载体本身内或由质粒携带的。使用丝氨酸-甘氨酸（SGGG）接头序列的多次重复，可以用带有基本C端肽序列（RRASV; cAMP依赖性蛋白激酶的靶位点）的修饰gpV进行高水平的野生型gpV取代。高度纯化的带有gpV-RRASV拷贝的噬菌体可以被适当的蛋白激酶有效地磷酸化，并显示掺入的标记仅在蛋白凝胶中以预期的大小迁移。再次使用Ser-Gly接头，可以将大的四聚体蛋白（β-半乳糖苷酶）以至少一个拷贝掺入活性病毒体中。这些研究表明，通过合适的间隔连接子和受控的表达水平，很可能在gpV的C末端融合了多种蛋白质或肽取代基，并将其组装成尾管的组成部分。 [参考：33]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号