• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >THE STRUCTURE OF AN RNA KISSING HAIRPIN COMPLEX OF THE HIV TAR HAIRPIN LOOP AND ITS COMPLEMENT
【24h】

THE STRUCTURE OF AN RNA KISSING HAIRPIN COMPLEX OF THE HIV TAR HAIRPIN LOOP AND ITS COMPLEMENT

机译：HIV TAR发夹环的含RNA的发夹复合物的结构及其补体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have used nuclear magnetic resonance (NMR) to obtain the structure of an RNA ''kissing'' hairpin complex formed between the HIV-2 TAR hairpin loop and a hairpin with a complementary loop sequence. Kissing hairpins are important in natural antisense reactions; their complex is a specific target for protein binding. The complex has all six nucleotides of each loop paired to form a bent quasicontinuous helix of three coaxially stacked helices: two stems plus a loop-loop interaction helix. Experimental constraints derived from heteronuclear and homonuclear NMR data on C-13 and N-15-labeled RNA led to a structure for the loop-loop helix with an average root-mean-square deviation of 0.83 (+/-0.10) A for 33 converged structures relative to the average structure. The loop-loop helix of the kissing complex is distorted compared to A-form RNA. Its major groove is blocked by the phosphodiester bonds that connect the first loop residue of each hairpin with its own stem, and it is flanked two negatively charged phosphate clusters. The loop-loop helix has alternating helical twists between adjacent base-pairs. The base-pairs at the helix junctions are overwound and three base-pairs near the helix junctions adopt high propeller twists. All these changes reduce the distance needed for the bridging phosphodiester bends connecting each stem and loop to cross the major groove of the loop-loop helix, and result in a deformed RNA helix with localized perturbations in the minor groove surface. The alternating helical tu ist pattern, plus other distortions in the loop-loop helix may be important for Rom protein recognition of the kissing hairpin complex. (C) Academic Press Limited. [References: 60]
机译：我们已经使用核磁共振（NMR）来获得在HIV-2 TAR发夹环和具有互补环序列的发夹之间形成的RNA“亲吻”发夹复合体的结构。亲吻发夹在自然反义反应中很重要。它们的复合物是蛋白质结合的特定靶标。该复合物具有每个环的所有六个核苷酸，形成三个同轴堆叠的螺旋的弯曲准连续螺旋：两个茎加一个环-环相互作用螺旋。从C-13和N-15标记的RNA的异核和同核NMR数据得出的实验约束导致33的环-环螺旋结构的平均均方根偏差为0.83（+/- 0.10）A相对于平均结构的收敛结构。与A型RNA相比，接吻复合物的环-环螺旋变形。它的主要沟被磷酸二酯键封闭，该磷酸二酯键将每个发夹的第一个环残基与自己的茎相连，并且其侧翼是两个带负电荷的磷酸盐簇。环-环螺旋在相邻碱基对之间具有交替的螺旋扭曲。螺旋连接处的碱基对被绕过，螺旋连接处附近的三个碱基对采用高螺旋桨扭曲。所有这些变化减少了连接每个茎和环的桥接磷酸二酯弯曲所需的距离，以跨越环-环螺旋的主要凹槽，并导致变形的RNA螺旋在较小凹槽表面具有局部扰动。交替的螺旋形扭纹图案，以及环-环螺旋中的其他变形对于Rom蛋白识别接吻发夹复合物可能很重要。 （C）学术出版社有限公司。 [参考：60]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号