• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Recognition of core binding sites by bacteriophage integrases
【24h】

Recognition of core binding sites by bacteriophage integrases

机译：通过噬菌体整合识别核心结合位点

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacteriophage integrases promote recombination between DNA molecules that carry attachment sites. They are members of a large and widely distributed family of site-specific recombinases with diverse biological roles. The integrases of phages lambda and HK022 are closely related members' of this family, but neither protein efficiently recombines the attachment sites of the other phage. The nucleotides responsible for this specificity difference are located close to the points of recombinational strand exchange, within an integrase binding motif called the extended core binding site. There are four imperfectly repeated copies of this motif in each set of phage attachment sites, but only two, B' and C, contain major specificity determinants. When these specificity determinants were replaced by the corresponding nucleotides from a site with the alternative specificity, the resulting mutant was recombined by both integrases. Thus, the determinants act by impeding recombination promoted by the non-cognate integrase. We found that identical nucleotide substitutions within different core site copies had different effects on recombination, suggesting that integrase does not recognize each of the extended core binding sites in the same way. Finally, substitution at several positions in lambda integrase with the corresponding HK022-specific amino acids prevents recombination of lambda attachment sites, and this defect can be suppressed in an allele-specific manner by appropriate substitutions of HK022-specific nucleotides in the extended core binding sites. (C) 1998 Academic Press Limited. [References: 36]
机译：噬菌体整合促进携带附着位点的DNA分子之间的重组。它们是具有广泛生物学作用的广泛且广泛分布的位点特异性重组酶家族的成员。 λ和HK022噬菌体的整合是该家族的密切相关成员，但是两种蛋白质均不能有效地重组另一噬菌体的附着位点。负责该特异性差异的核苷酸位于被称为延伸核心结合位点的整合酶结合基序内，靠近重组链交换点。在每组噬菌体附着位点中，该基序有四个不完全重复的副本，但是只有两个B'和C包含主要的特异性决定子。当这些特异性决定簇被来自具有替代特异性的位点的相应核苷酸替换时，所得突变体通过两个整合重组。因此，决定簇通过阻止由非同源整合酶促进的重组而起作用。我们发现不同核心位点副本内相同的核苷酸取代对重组具有不同的影响，表明整合酶不能以相同方式识别每个扩展的核心结合位点。最后，在lambda整合酶中的几个位置上替换相应的HK022特异性氨基酸可防止lambda附着位点重组，并且可以通过等位基因特异性方式通过在扩展的核心结合位点中适当替换HK022特异性核苷酸来抑制该缺陷。 （C）1998 Academic Press Limited。 [参考：36]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号