...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >NUCLEOSOME DNA SEQUENCE PATTERN REVEALED BY MULTIPLE ALIGNMENT OF EXPERIMENTALLY MAPPED SEQUENCES
【24h】

NUCLEOSOME DNA SEQUENCE PATTERN REVEALED BY MULTIPLE ALIGNMENT OF EXPERIMENTALLY MAPPED SEQUENCES

机译：通过实验映射的序列的多重校正揭示了核糖体DNA序列模式

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Five different algorithms have been applied for detecting DNA sequence pattern hidden in 204 DNA sequences collected from the literature which are experimentally found to be involved in nucleosome formation. Each algorithm was used to perform a multiple alignment of the nucleosome DNA sequences within the window 145 nt, the size of a nucleosome core DNA. From these alignments five pairs of AA and TT dinucleotide positional frequency distributions have been computed. The frequency profiles calculated by different algorithms are rather different due to substantial noise. They, however, share several important features. Both AA and TT dinucleotide positional frequencies display periodicity with the period of 10.3(+/-0.2) bases. TT dinucleotides appear to be distributed symmetrically relative to AA dinucleotides of the same DNA strand, with the center of symmetry at the midpoint of the nucleosome core DNA. The phase shift between the AA and TT patterns is about 6 bp. Superposition of the five pairs of the AA (TT) positional frequency profiles has produced the refined pattern, with the above features well pronounced. An interesting novel feature of the pattern is an absence of central peaks in the periodical AA and TT distributions. This may indicate that the central section of nucleosome DNA, 15 bp around the dyad axis of the nucleosome, is not bent. Positional distributions of other dinucleotides were not found in this study to be as informative as the ones for AA and TT. (C) 1996 Academic Press Limited [References: 42]
机译：已经应用了五种不同的算法来检测隐藏在从文献中收集的204个DNA序列中的DNA序列模式，这些序列在实验中被发现与核小体形成有关。使用每种算法对窗口145 nt（核小体核心DNA的大小）内的核小体DNA序列进行多重比对。从这些比对中，已经计算出五对AA和TT二核苷酸的位置频率分布。由于存在大量噪声，因此由不同算法计算出的频率曲线差异很大。但是，它们具有几个重要功能。 AA和TT二核苷酸的位置频率都显示周期为10.3（+/- 0.2）个碱基的周期。 TT二核苷酸似乎相对于同一DNA链的AA二核苷酸对称分布，对称中心位于核小体核心DNA的中点。 AA和TT模式之间的相移约为6 bp。五对AA（TT）位置频率曲线的叠加产生了精确的图案，上述特征非常明显。该模式的一个有趣的新颖特征是在周期AA和TT分布中不存在中心峰。这可能表明核小体DNA的中心部分（围绕核小体的dyad轴约15 bp）没有弯曲。在这项研究中没有发现其他二核苷酸的位置分布与AA和TT一样丰富。 （C）1996 Academic Press Limited [参考号：42]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号