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首页> 外文期刊>Journal of Molecular Biology >THE NUIA GENE FROM ANABAENA SP. ENCODING AN INHIBITOR OF THE NUCA SUGAR-NON-SPECIFIC NUCLEASE
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THE NUIA GENE FROM ANABAENA SP. ENCODING AN INHIBITOR OF THE NUCA SUGAR-NON-SPECIFIC NUCLEASE

机译：来自ANABAENA SP的NUIA基因。编码NUCA糖非特异性核酸酶抑制剂

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摘要

Many filamentous, heterocyst-forming cyanobacteria express a sugar-nonspecific nuclease of about 29 kDa that can be detected in DNA-containing SDS-PAGE gels. The nucA gene encoding this nuclease has previously been cloned from Anabaena sp. PCC 7120, sequenced and expressed in Escherichia cell. The NucA protein bears a putative signal peptide close to its N-terminal end and, in Anabaena cultures, is present in both the cells and the extracellular medium. Cell-free extracts of different cyanobacteria producing NucA-like nucleases exhibited an inhibitory activity on NucA. Ln Anabaena sp. PCC 7120, this inhibition was exerted by protein(s) or protein-containing molecule(s) that were heat resistant. Immediately downstream from the nucA gene, in the complementary strand, we have identified an open reading frame composed of 135 codons, that we have named nuiA, whose expression in E. coli conferred heat-resistant NucA-inhibitory activity to cell-free extracts. The NuiA protein was purified to homogeneity, and purified NuiA inhibited the nuclease activity of NucA. Sequences hybridizing with the nuiA gene have been found in all the tested cyanobacterial strains that express a NucA-like nuclease. Whereas the NucA protein is homologous to endonuclease G from vertebrates and to nucleases from Serratia marcescens and yeast, no protein homologous to NuiA was found in the available databases. Therefore, nuiA represents a novel gene encoding a nuclease inhibitor. (C) 1997 Academic Press Limited. [References: 25]
机译：许多丝状，形成异囊的蓝细菌表达的糖非特异性核酸酶约为29 kDa，可在含DNA的SDS-PAGE凝胶中检测到。先前已从鱼腥藻（Anabaena sp。）克隆了编码该核酸酶的nucA基因。在大肠杆菌细胞中测序并表达的PCC 7120。 NucA蛋白在其N末端附近带有推定的信号肽，在鱼腥藻培养物中，它同时存在于细胞和细胞外培养基中。产生NucA样核酸酶的不同蓝藻的无细胞提取物表现出对NucA的抑制活性。 Ln鱼腥藻PCC 7120，这种抑制作用是由耐热的蛋白质或含蛋白质的分子施加的。在互补链中，紧接nucA基因的下游，我们已经确定了一个由135个密码子组成的开放阅读框，我们将其命名为nuiA，其在大肠杆菌中的表达赋予无细胞提取物耐热的NucA抑制活性。将NuiA蛋白纯化至均质，并且纯化的NuiA抑制NucA的核酸酶活性。已经在所有测试的表达NucA样核酸酶的蓝细菌菌株中发现了与nuiA基因杂交的序列。 NucA蛋白与脊椎动物的核酸内切酶G同源，与粘质沙雷氏菌和酵母的核酸酶同源，但在可用数据库中未发现与NuiA同源的蛋白。因此，nuiA代表编码核酸酶抑制剂的新基因。 （C）1997 Academic Press Limited。 [参考：25]

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