Dual mode of interaction of DNA polymerase epsilon with proliferating cell nuclear antigen in primer binding and DNA synthesis.

Maga G; Jonsson ZO; Stucki M; Spadari S; Hubscher U

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Dual mode of interaction of DNA polymerase epsilon with proliferating cell nuclear antigen in primer binding and DNA synthesis.

机译：DNA聚合酶epsilon与增殖细胞核抗原在引物结合和DNA合成中的双重相互作用模式。

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Proliferating cell nuclear antigen can interact with DNA polymerase epsilon on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication protein A), and thereby stimulate its primer recognition and DNA synthesis. Using four characterized mutants of proliferating cell nuclear antigen containing three or four alanine residue substitutions on the C-terminal side and the back side of the trimer, we have tested the kinetics of primer binding and nucleotide incorporation by DNA polymerase epsilon in different assays. In contrast with what has been found in interaction studies between DNA polymerase delta and proliferating cell nuclear antigen, our data suggested that stimulation of DNA polymerase epsilon primer binding involves interactions with both the C-terminal side and the back side of proliferating cell nuclear antigen. However, for stimulation of DNA polymerase epsilon DNA synthesis, exclusively the C-terminal side appears to be sufficient. The significance of this dual interaction is discussed with reference to the physiological roles of DNA polymerase epsilon and its interaction with the clamp proliferating cell nuclear antigen. Copyright 1999 Academic Press.

机译：即使在没有其他辅助因子（复制因子C，复制蛋白A）的情况下，增殖细胞核抗原也可以与线性DNA模板上的DNA聚合酶ε相互作用，从而刺激其引物识别和DNA合成。使用在三聚体的C端侧和背面含有三个或四个丙氨酸残基取代的增殖细胞核抗原的四个特征突变体，我们已经在不同的试验中测试了引物结合和DNA聚合酶ε核苷酸掺入的动力学。与在DNA聚合酶δ和增殖细胞核抗原之间的相互作用研究中发现的相反，我们的数据表明，DNA聚合酶ε引物结合的刺激涉及与增殖细胞核抗原的C端和背面的相互作用。然而，对于刺激DNA聚合酶εDNA的合成，仅C末端侧似乎是足够的。参照DNA聚合酶epsilon的生理作用及其与夹具增殖细胞核抗原的相互作用，讨论了这种双重相互作用的意义。版权所有1999，学术出版社。

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《Journal of Molecular Biology》 |1999年第1期|共9页
作者
Maga G; Jonsson ZO; Stucki M; Spadari S; Hubscher U;
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正文语种 eng
中图分类分子生物学;
关键词
DNA; DNA Polymerase II; Proliferating Cell Nuclear Antigen; DNA Polymerase III; DNA聚合酶Ⅱ; 增殖细胞核抗原;

机译：DNA;DNA Polymerase II;Proliferating Cell Nuclear Antigen;DNA Polymerase III;DNA聚合酶Ⅱ;增殖细胞核抗原;

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专利

1. Dual mode of interaction of DNA polymerase epsilon with proliferating cell nuclear antigen in primer binding and DNA synthesis. [J] . Maga G, Jonsson ZO, Stucki M, Journal of Molecular Biology . 1999,第1期

机译：DNA聚合酶epsilon与增殖细胞核抗原在引物结合和DNA合成中的双重相互作用模式。
2. DNA polymerase epsilon interacts with proliferating cell nuclear antigen in primer recognition and elongation. [J] . Maga G, Hubscher U Biochemistry . 1995,第3期

机译：DNA聚合酶ε与引物识别和延伸中的增殖细胞核抗原相互作用。
3. THE MAMMALIAN DNA POLYMERASE DELTA-PROLIFERATING CELL NUCLEAR ANTIGEN-TEMPLATE-PRIMER COMPLEX - MOLECULAR CHARACTERIZATION BY DIRECT BINDING [J] . Mcconnell M, Miller H, Mozzherin DJ, Biochemistry . 1996,第25期

机译：哺乳动物DNA聚合酶δ增生细胞核抗原-模板底物复合物的直接结合表征。
4. Towards an understanding of protein-protein interaction network hierarchies. Analysis of DnaN (β)-binding peptide motifs in members of protein families interacting with the eubacterial processivity clamp, the β subunit of DNA Polymerase III [C] . Brian P. Dalrymple, Gene Wijffels, Kritaya Kongsuwan, Asia-Pacific Bioinformatics Conference(APBC 2003); 200302; Adelaide(AU) . 2003

机译：对蛋白质-蛋白质相互作用网络层次结构的理解。蛋白质家族成员与DNA聚合酶III的优生过程性钳位相互作用的DnaN（β）结合肽基序分析
5. C-terminal flap endonuclease (rad27) mutations: Lethal interactions with a DNA ligase I mutation (cdc9-p) and suppression by proliferating cell nuclear antigen (POL30) in Saccharomyces cerevisiae. [D] . Karanja, Kenneth Kimani. 2009

机译：C末端皮瓣内切核酸酶（rad27）突变：与酿酒酵母中DNA连接酶I突变（cdc9-p）的致命相互作用以及通过增殖细胞核抗原（POL30）的抑制作用。
6. Mutations in yeast proliferating cell nuclear antigen define distinct sites for interaction with DNA polymerase delta and DNA polymerase epsilon. [O] . J C Eissenberg, R Ayyagari, X V Gomes, 1997

机译：酵母增殖细胞核抗原中的突变定义了与DNA聚合酶δ和DNA聚合酶ε相互作用的不同位点。
7. Mutations in yeast proliferating cell nuclear antigen define distinct sites for interaction with DNA polymerase delta and DNA polymerase epsilon. [O] . Eissenberg, J C, Ayyagari, R, Gomes, X V, 1997

机译：酵母增殖细胞核抗原中的突变定义了与DNA聚合酶δ和DNA聚合酶ε相互作用的不同位点。

Dual mode of interaction of DNA polymerase epsilon with proliferating cell nuclear antigen in primer binding and DNA synthesis.

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