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首页> 外文期刊>Journal of Molecular Biology >EXCLUSIVE EXPRESSION OF C-ELEGANS OSM-3 KINESIN GENE IN CHEMOSENSORY NEURONS OPEN TO THE EXTERNAL ENVIRONMENT
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EXCLUSIVE EXPRESSION OF C-ELEGANS OSM-3 KINESIN GENE IN CHEMOSENSORY NEURONS OPEN TO THE EXTERNAL ENVIRONMENT

机译：C-elegans OSM-3驱动蛋白基因在外部环境开放的化学传感神经元中的排他性表达

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摘要

In Caenorhabditis elegans three genetic loci osm-3, unc-104 and unc-116 have been identified, which encode anterograde motor kinesin. Here we show that osm-3 encodes a 672 amino acid long kinesin-like protein (KLP) that contains all three functional domains similar to the kinesin heavy chain, including a globular motor region, an alpha-helical coiled-coil rod, and a globular tail region. OSM-3 shows homology in both the motor and rod domains with kinesins from divergent species such as mouse KIF3, and sea urchin KRP95, and also with the rod domains of several non-kinesin proteins, such as myosin, ezrin, outer membrane proteins alpha precursor OMPA, yeast intracellular protein transport USO1, and the rat neurofilament NF-H. Temporal and spatial expression of the osm-3::lacZ fusion gene during development is limited to an exclusive set of 26 chemosensory neurons whose dendritic endings are exposed to the external environment, including six IL2 neurons of the inner labial sensilla, eight pairs of amphid neurons (ADE ADL, ASE, ASG, ASH, ASI, ASJ, ASK) in the head, and two pairs of phasmid neurons (PHA and PHB) in the tail. Our data are consistent with the known structural defects in the amphid and phasmid sensilla in osm-3 mutants and also show the expression of the gene in IL2 neurons. Temporally, the gene is differentially expressed in all three types of chemosensory sensilla. Further work on osm-3, unc-104 and unc-116 mutants should give insight into the in vivo functions of the kinesin family during C. elegans neurogenesis. [References: 80]
机译：在秀丽隐杆线虫中，已经鉴定出三个基因座osm-3，unc-104和unc-116，它们编码顺行运动驱动蛋白。在这里，我们显示osm-3编码一个672个氨基酸的长驱动蛋白样蛋白（KLP），该蛋白包含与驱动蛋白重链相似的所有三个功能域，包括一个球形运动区，一个α螺旋盘绕棒和一个球形尾区。 OSM-3在运动和杆域中均与来自不同物种的驱动蛋白如小鼠KIF3和海胆KRP95同源，还与几种非驱动蛋白的杆域（如肌球蛋白，ezrin，外膜蛋白α）同源。 OMPA前体，酵母细胞内蛋白转运USO1和大鼠神经丝NF-H。 osm-3 :: lacZ融合基因在发育过程中的时空表达仅限于26个化学感应神经元的专有集合，这些神经元的树突末梢暴露于外部环境，包括内唇感觉器的6个IL2神经元，8对两性头部神经元（ADE ADL，ASE，ASG，ASH，ASI，ASJ，ASK），尾巴中有两对成虫（PHA和PHB）。我们的数据与已知的osm-3突变体中的两性和噬菌体感官结构缺陷一致，并且还显示了该基因在IL2神经元中的表达。暂时地，该基因在所有三种类型的化学感觉传感器中差异表达。有关osm-3，unc-104和unc-116突变体的进一步工作应使我们深入了解秀丽隐杆线虫神经发生过程中驱动蛋白家族的体内功能。 [参考：80]

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