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首页> 外文期刊>Journal of Molecular Biology >Characterization of loose and tight dimer forms of avian leukosis virus RNA.
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Characterization of loose and tight dimer forms of avian leukosis virus RNA.

机译：禽白血病病毒RNA的松散和紧密二聚体形式的表征。

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摘要

Retroviral genomes consist of two identical RNA molecules joined non-covalently near their 5'-ends. Recently, we showed that an imperfect autocomplementary sequence, located in the L3 domain, plays an essential role in avian sarcoma-leukosis virus (ASLV) RNA dimerization in vitro. This sequence can adopt a stem-loop structure and is involved in ASLV replication. Here, we found that in the absence of nucleocapsid protein, RNA transcripts of avian leukosis virus (ALV) were able to form two types of dimers in vitro that differ in their stability: a loose dimer, formed at a physiological temperature, and a tight dimer, formed at a high temperature. A mutational analysis was performed to define the features of these dimers. The results of this analysis unambiguously confirm that the two L3 stem-loops interact directly in both types of dimers. A loop-loop interaction is the main linkage in the loose dimer. In contrast, in the tight dimer, the stem and the loop of the L3 hairpin form an extended duplex. Surprisingly, we also found that the dimerization properties defined for our ALV strain (type SR-A) differ from those found in other ASLV strains. Copyright 2000 Academic Press.
机译：逆转录病毒基因组由两个相同的RNA分子组成，它们的5'端附近非共价连接。最近，我们显示，位于L3结构域中的不完善的自身互补序列在禽肉瘤白血病病毒（ASLV）RNA二聚体体外起着至关重要的作用。该序列可以采用茎环结构，并参与ASLV复制。在这里，我们发现在缺乏核衣壳蛋白的情况下，禽白血病病毒（ALV）的RNA转录物能够在体外形成两种类型的二聚体，它们的稳定性有所不同：在生理温度下形成的松散二聚体和紧密的二聚体。在高温下形成的二聚体。进行了突变分析以定义这些二聚体的特征。该分析的结果明确证实了两个L3茎环在两种类型的二聚体中直接相互作用。环-环相互作用是疏松二聚体中的主要联系。相反，在紧密二聚体中，L3发夹的茎和环形成延伸的双链体。出乎意料的是，我们还发现，为我们的ALV菌株（SR-A型）定义的二聚化性质不同于其他ASLV菌株中发现的二聚化性质。版权所有2000学术出版社。

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