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首页> 外文期刊>Journal of Molecular Biology >STRUCTURE AND MECHANISM OF A SUB-FAMILY OF ENZYMES RELATED TO N-ACETYLNEURAMINATE LYASE
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STRUCTURE AND MECHANISM OF A SUB-FAMILY OF ENZYMES RELATED TO N-ACETYLNEURAMINATE LYASE

机译：N-乙酰神经氨酸裂解酶相关的酶亚家族的结构和机制

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摘要

We describe here a sub-family of enzymes related both structurally and functionally to N-acetylneuraminate lyase. Two members of this family (N-acetylneuraminate lyase and dihydrodipicolinate synthase) have known three-dimensional structures and we now proceed to show their structural and functional relationship to two further proteins, trans-o-hydroxybenzylidenepyruvate hydratase-aldolase and D-4-deoxy-5-oxoglucarate dehydratase. These enzymes are all thought to involve intermediate Schiff-base formation with their respective substrates. In order to understand the nature of this intermediate, we have determined the three-dimensional structure of N-acetylneuraminate lyase in complex with hydroxypyruvate (a product analogue) and in complex with one of its products (pyruvate). From these structures we deduce the presence of a closely similar Schiff-base forming motif in all members of the N-acetylneuraminate lyase sub-family. A fifth protein, MosA, is also confirmed to be a member of the sub-family although the involvement of an intermediate Schiff-base in its proposed reaction is unclear. (C) 1997 Academic Press Limited. [References: 68]
机译：我们在这里描述了与N-乙酰神经氨酸裂合酶在结构和功能上都相关的酶的一个亚家族。该家族的两个成员（N-乙酰神经氨酸裂解酶和二氢二吡啶甲酸合酶）具有三维结构，我们现在继续显示它们与另外两种蛋白质的结构和功能关系，即反式-o-羟基苄叉基丙酮酸水合酶-醛缩酶和D-4-脱氧酶-5-氧葡萄糖酸脱水酶。这些酶都被认为涉及中间的席夫碱形成及其各自的底物。为了了解这种中间体的性质，我们确定了N-乙酰神经氨酸裂解酶与羟基丙酮酸（一种产物类似物）的复合物以及与其一种产物（丙酮酸）的复合物的三维结构。从这些结构，我们推论在N-乙酰神经氨酸裂解酶亚家族的所有成员中都存在相似的席夫碱形成基序。尽管尚不清楚中间席夫碱是否参与了其拟议的反应，但也证实了第五种蛋白MosA是该亚家族的成员。 （C）1997 Academic Press Limited。 [参考：68]

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