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首页> 外文期刊>Journal of Molecular Biology >DIMER FORM OF PHOSPHORYLATED SPO0A, A TRANSCRIPTIONAL REGULATOR, STIMULATES THE SPO0F TRANSCRIPTION AT THE INITIATION OF SPORULATION IN BACILLUS SUBTILIS
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DIMER FORM OF PHOSPHORYLATED SPO0A, A TRANSCRIPTIONAL REGULATOR, STIMULATES THE SPO0F TRANSCRIPTION AT THE INITIATION OF SPORULATION IN BACILLUS SUBTILIS

机译：磷酸化SPO0A的二聚体形式，一种转录调节剂，在芽胞杆菌芽孢形成初期刺激SPO0F转录。

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摘要

The SpoOA protein of Bacillus subtilis is a transcriptional regulator that shows extensive homology to the regulator proteins in bacterial two-component regulatory systems. Phosphorylation of SpoOA is absolutely necessary for the initiation of sporulation. We now show that phospho-SpoOA is a dimer, binds specifically to the spoOF promoter region, and stimulates the transcription from the P2 promoter recognized by sigma(H)-RNA polymerase. Biochemical and biological analyses suggest that phospho-SpoOA interacts directly with the ''OA-like box'' sequence (TGTCGTA) located in the spoOF promoter region. Phosphorylation of SpoOA enhanced its affinity to the OA-like box. Evidence is also presented that the spoOF promoter region contains a static bend having two sets of oligo(dA-dT) tracts. It was demonstrated that the bending region overlaps with the recognition site for the phospho-SpoOA. [References: 51]
机译：枯草芽孢杆菌的SpoOA蛋白是一种转录调节因子，与细菌两成分调节系统中的调节蛋白具有广泛的同源性。 SpoOA的磷酸化对于孢子形成的启动是绝对必要的。现在，我们显示磷酸SpoOA是一个二聚体，与spoOF启动子区域特异性结合，并刺激由sigma（H）-RNA聚合酶识别的P2启动子的转录。生化和生物学分析表明，磷酸化SpoOA与位于spoOF启动子区域的“ OA样盒”序列（TGTCGTA）直接相互作用。 SpoOA的磷酸化增强了其对OA样盒的亲和力。还提供了证据，即spoOF启动子区域包含具有两组寡聚（dA-dT）道的静态弯曲。已证明弯曲区域与磷酸-SpoOA的识别位点重叠。 [参考：51]

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