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首页> 外文期刊>Journal of Molecular Biology >SUBSTRATE PREFERENCES OF VSR DNA MISMATCH ENDONUCLEASE AND THEIR CONSEQUENCES FOR THE EVOLUTION OF THE ESCHERICHIA COLI K-12 GENOME
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SUBSTRATE PREFERENCES OF VSR DNA MISMATCH ENDONUCLEASE AND THEIR CONSEQUENCES FOR THE EVOLUTION OF THE ESCHERICHIA COLI K-12 GENOME

机译：VSR DNA错配核糖核酸酶的基质表达及其对大肠杆菌K-12基因组进化的影响

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摘要

The substrate spectrum of Vsr DNA mismatch endonuclease of Escherichia coli K-12 was investigated using fluorescence-labelled oligonucleotide substrates and a DNA sequencer for detection and quantification of substrates and reaction products. Fourteen substrates were found to be processed by the enzyme, which differ in one or two positions from the canonical pentanucleotide sequence CTA/(T)GG (T mismatched to G). Relative second-order rate constants of these substrates were determined in groups of four by multiple substrate kinetics and compared to the underrepresentation of the corresponding pentanucleotides in the E. coli K-12 genome. The high quality of correlation further establishes active mutagenesis by VSP repair as a significant driving force of the evolution of the E. coli K-12 genome and provides clues to its possible selective value. [References: 22]
机译：使用荧光标记的寡核苷酸底物和DNA测序仪检测和定量底物和反应产物，研究了大肠杆菌K-12的Vsr DNA错配核酸内切酶的底物光谱。发现十四种底物被该酶处理，其底物与规范的五核苷酸序列CTA /（T）GG（T与G不匹配）相差一两个位置。这些底物的相对二级速率常数由多个底物动力学确定为四个一组，并与大肠杆菌K-12基因组中相应五核苷酸的代表性不足进行比较。高质量的相关性进一步通过VSP修复建立了主动诱变作用，将其作为大肠杆菌K-12基因组进化的重要驱动力，并为其潜在的选择价值提供了线索。 [参考：22]

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