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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Preliminary atomic force microscopy study of two-dimensional crystals of lactose permease from Escherichia coli.
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Preliminary atomic force microscopy study of two-dimensional crystals of lactose permease from Escherichia coli.

机译：大肠杆菌乳糖渗透酶二维晶体的初步原子力显微镜研究。

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摘要

Lactose permease (LacY) of Escherichia coli is not only a paradigm for secondary transporters but also for difficulties in two-dimensional (2D) crystallization. In this work we present the progresses achieved in the observation of 2D crystals of wild-type LacY by atomic force microscopy (AFM). Crystals were obtained following reconstitution of LacY in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes. Proteolipid sheets (PLSs) 6.4 nm in height were obtained after spreading the samples onto mica. Observations were carried out in liquid medium and in contact mode (CM-AFM). When the crystalline surfaces of the PLSs were imaged regular packing arrangements were observed. The back-Fourier transformation revealed the existence of various orientations mostly consistent with crystals possessing p2 symmetry and unit-cell dimensions: a=13.15 nm, b=16.74 nm, gamma=116 degrees. The characteristics, size, and shape of the repetitive motif could be compatible with dimers of this protein. These preliminary results are compared and discussed with previously reported 2D crystals observed by electron microscopy.
机译：大肠杆菌的乳糖通透酶（LacY）不仅是二级转运蛋白的范例，而且还是二维（2D）结晶困难的原因。在这项工作中，我们介绍了通过原子力显微镜（AFM）观察野生型LacY二维晶体的研究进展。在1-棕榈酰基-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）脂质体内重构LacY后获得晶体。将样品铺展到云母上后，获得高度为6.4 nm的蛋白脂片（PLS）。在液体介质中和以接触模式（CM-AFM）进行观察。当对PLS的结晶表面成像时，观察到规则的堆积排列。傅里叶逆变换表明​​存在与具有p2对称性和晶胞尺寸的晶体基本一致的各种取向：a = 13.15 nm，b = 16.74 nm，γ= 116度。重复基序的特征，大小和形状可能与此蛋白的二聚体相容。将这些初步结果与先前通过电子显微镜观察到的二维晶体进行了比较和讨论。

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