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首页> 外文期刊>Journal of Neurosurgery. Spine. >Changes in pain behavior and glial activation in the spinal dorsal horn after pulsed radiofrequency current administration to the dorsal root ganglion in a rat model of lumbar disc herniation: Laboratory investigation
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Changes in pain behavior and glial activation in the spinal dorsal horn after pulsed radiofrequency current administration to the dorsal root ganglion in a rat model of lumbar disc herniation: Laboratory investigation

机译：腰椎间盘突出症大鼠模型中脉冲射频电流施加到背根神经节后，疼痛行为和脊髓背角神经胶质激活的变化

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Object. Herniated discs can induce sciatica by mechanical compression and/or chemical irritation caused by proinflammatory cytokines. Using immunohistochemistry methods in the dorsal horn of a rat model of lumbar disc herniation, the authors investigated the effects of pulsed radiofrequency (PRF) current administration to the dorsal root ganglion (DRG) on pain-related behavior and activation of microglia, astrocytes, and mitogen-activated protein kinase. Methods. A total of 33 Sprague-Dawley rats were randomly assigned to either a sham-operated group (n = 10) or a nucleus pulposus (NP)-exposed group (n = 23). Rats in the NP-exposed group were further subdivided into NP exposed with sham stimulation (NP+sham stimulation, n = 10), NP exposed with PRF (NP+PRF, n = 10), or euthanasia 10 days after NP exposure (n = 3). The DRGs in the NP+PRF rats were exposed to PRF waves (2 Hz) for 120 seconds at 45 V on postoperative Day 10. Rats were tested for mechanical allodynia 10 days after surgery and at 8 hours, 1 day, 3 days, 10 days, 20 days, and 40 days after PRF administration. Immunohistochemical staining of astrocytes (glial fibrillary acidic protein), microglia (OX-42), and phosphorylated extracellular signal-regulated kinases (pERKs) in the spinal dorsal horn was performed at 41 days after PRF administration. Results. Starting at 8 hours after PRF administration, mechanical withdrawal thresholds dramatically increased; this response persisted for 40 days (p < 0.05). After PRF administration, immunohistochemical expressions of OX-42 and pERK in the spinal dorsal horn were quantitatively reduced (p < 0.05). Conclusions. Pulsed radiofrequency administration to the DRG reduced mechanical allodynia and downregulated microglia activity and pERK expression in the spinal dorsal horn of a rat model of lumbar disc herniation. .
机译：目的。椎间盘突出可以通过由促炎性细胞因子引起的机械压迫和/或化学刺激来诱导坐骨神经痛。作者在大鼠腰椎间盘突出症模型的背角中使用免疫组织化学方法，研究了脉冲射频（PRF）电流对背根神经节（DRG）的给药对疼痛相关行为和小胶质细胞，星形胶质细胞和神经胶质细胞活化的影响。丝裂原激活的蛋白激酶。方法。将总共​​33只Sprague-Dawley大鼠随机分为假手术组（n = 10）或髓核（NP）暴露组（n = 23）。 NP暴露组的大鼠进一步分为假刺激（NP +假刺激，n = 10），PRF暴露（NP + PRF，n = 10）或NP暴露10天后的安乐死（n）。 = 3）。 NP + PRF大鼠的DRG在术后第10天以45 V电压暴露于PRF波（2 Hz）120秒，在术后10天和8小时，1天，3天，10天对大鼠进行机械性异常性疼痛测试PRF给药后的10天，20天和40天。施用PRF后41天，对脊髓背角中的星形胶质细胞（神经胶质纤维酸性蛋白），小胶质细胞（OX-42）和磷酸化的细胞外信号调节激酶（pERK）进行了免疫组织化学染色。结果。从PRF给药后的8小时开始，机械退出阈值急剧增加。这种反应持续了40天（p <0.05）。 PRF给药后，脊髓背角中OX-42和pERK的免疫组织化学表达被定量降低（p <0.05）。结论。对DRG进行脉冲射频管理可减少机械性异常性疼痛，并下调腰椎间盘突出症大鼠脊髓背角的小胶质细胞活性和pERK表达。 。

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