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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Low doses of alpha- and gamma-radiation enhance DNA thermal stability.
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Low doses of alpha- and gamma-radiation enhance DNA thermal stability.

机译：低剂量的α和γ辐射可增强DNA的热稳定性。

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摘要

Isolated calf thymus DNA in buffered solutions has been exposed to 0-150 Gy of alpha- and gamma-radiation. The effects of alpha- and gamma-radiation on the thermal stability and electrophoretic mobility of the DNA molecules have been studied by UV spectroscopic 'melting' and Pulsed Field Gel Electrophoresis (PFGE), respectively. The observed thermal denaturation parameters were fitted to the energy propagation descriptive model. The experimental results for the samples exposed to relatively low (low) doses indicate an increased thermal stability and a reduced mobility over that of the controls. The expected overall degradation of the DNA molecules was confirmed for the samples exposed to high doses. Our results are in good agreement with the predictions of the energy propagation model, which now is also tested in the low dose region and for an additional type of ionising radiation (alpha-particles). Our findings are consistent with conformational changes at low doses resulting in a DNA form characterised by localised alterations, which affect the energy flow along the DNA molecule.
机译：缓冲溶液中分离出的小牛胸腺DNA已暴露于0-150 Gy的α和γ辐射。分别通过紫外光谱“熔融”和脉冲场凝胶电泳（PFGE）研究了α和γ辐射对DNA分子的热稳定性和电泳迁移率的影响。将观察到的热变性参数拟合到能量传播描述模型中。暴露于相对低（低）剂量的样品的实验结果表明，与对照相比，其热稳定性提高，迁移率降低。对于暴露于高剂量的样品，确认了预期的DNA分子整体降解。我们的结果与能量传播模型的预测非常吻合，该模型现在也已在低剂量区域和其他类型的电离辐射（α粒子）中进行了测试。我们的发现与低剂量的构象变化相一致，从而导致以局部改变为特征的DNA形式，从而影响沿DNA分子的能量流动。

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