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首页> 外文期刊>Journal of neuroendocrinology >Prolactin and oxytocin interaction in the paraventricular and supraoptic nuclei: effects on oxytocin mRNA and nitric oxide synthase.
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Prolactin and oxytocin interaction in the paraventricular and supraoptic nuclei: effects on oxytocin mRNA and nitric oxide synthase.

机译：催乳素和催产素在室旁和视上核中的相互作用：对催产素mRNA和一氧化氮合酶的影响。

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摘要

We investigated the contribution of prolactin and oxytocin to the increase in staining for NADPH-d and oxytocin mRNA in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) observed at the end of pregnancy, or following a steroid-priming regimen that mimics the hormonal profile of late pregnant females. Ovariectomized rats received chronic implants of silastic capsules containing oestrogen and progesterone followed by progesterone removal. In experiment 1, oxytocin antagonist (OTA) was administered to rats to investigate whether intranuclear oxytocin release was necessary for NADPH-d staining. In experiments 2a and b, rats received concurrent treatment with bromocryptine (0.5 mg/day) to suppress endogenous prolactin release, and either systemic prolactin (0.5 mg once daily), or prolactin (2 micro g/ micro l), or vehicle infused twice a day into the third ventricle, or chronic oxytocin infusion (24 ng/day) for 3 days following progesterone removal. Brains were then processed for NADPH-d histochemistry. In experiment 3, the interaction of prolactin and oxytocin on oxytocin mRNA within the SON and PVN was examined. NADPH-d staining in the SON and PVN was reduced by the highest dose of the OTA, and by bromocryptine treatment. Central prolactin and oxytocin replacement completely restored NADPH-d staining in bromocryptine-treated rats. Finally, both bromocryptine and the OTA suppressed oxytocin mRNA expression and prolactin replacement restored expression levels to that of controls. Together, these data suggest that the increased capacity to produce nitric oxide in the SON and PVN during late pregnancy is dependent on prolactin stimulating oxytocin gene mRNA and hence intranuclear oxytocin release.
机译：我们研究了催乳素和催产素对妊娠末期或模拟孕激素的类固醇引发方案中脑室旁核（PVN）和视上核（SON）中NADPH-d和催产素mRNA染色增加的贡献。晚期孕妇的荷尔蒙概况。切除卵巢的大鼠接受了长期植入的含有雌激素和孕酮的硅质胶囊的植入，然后去除了孕激素。在实验1中，给大鼠施用催产素拮抗剂（OTA），以研究核内催产素的释放对于NADPH-d染色是否必要。在实验2a和b中，大鼠接受溴隐亭（0.5 mg /天）的同时治疗以抑制内源性催乳素的释放，并且全身性催乳素（0.5 mg /天）或催乳素（2 micro g / microl）或溶媒注入两次每天在第三脑室注射，或在去除孕激素后连续3天进行慢性催产素输注（24 ng /天）。然后对大脑进行NADPH-d组织化学处理。在实验3中，检查了催乳素和催产素对SON和PVN内催产素mRNA的相互作用。通过最大剂量的OTA以及溴隐汀处理可以降低SON和PVN中的NADPH-d染色。中央催乳素和催产素的替代完全恢复了溴隐汀治疗大鼠的NADPH-d染色。最后，溴隐隐素和OTA都抑制催产素mRNA的表达，催乳素替代使表达水平恢复到对照水平。总之，这些数据表明妊娠晚期在SON和PVN中产生一氧化氮的能力增加取决于催乳素刺激催产素基因mRNA，从而取决于核内催产素的释放。

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