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LOCAL ANALYSIS OF THE M STATE KINETICS OF BACTERIORHODOPSIN

机译:细菌视紫红质M态动力学的局部分析

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摘要

Two kinetic models (A and B) of the photocycle of bacteriorhodopsin are analyzed around the M state and compared with experimental kinetic absorption data measured at 410 nm. Both models assume two isospectral M states: M(1) and M(2). Model A is a sequential allowing back reaction between the L and M(1) intermediate states. Model B has a branching after L to M(1) and M(2). Solving the first order reaction differential equations it is shown that both models result in the same time dependence for the transient absorption change at 410 nm (M(1) + M(2) intermediate states). This time functional form describes well the experimental data. Though the two models cannot be distinguished on the basis of the functional form alone. But fitting to the experimental data gives acceptable parameters for model B; for model A we get unacceptable results. [References: 20]
机译:在M状态附近分析了细菌视紫红质光周期的两个动力学模型(A和B),并与在410 nm处测得的实验动力学吸收数据进行了比较。两种模型均假设两个等光谱M状态:M(1)和M(2)。模型A是顺序的,允许在L和M(1)中间状态之间发生逆反应。模型B在L到M(1)和M(2)之后具有分支。求解一阶反应微分方程表明,对于410 nm处的瞬态吸收变化(M(1)+ M(2)中间态),两个模型都导致相同的时间依赖性。这次的功能形式很好地描述了实验数据。尽管不能仅基于功能形式来区分这两种模型。但是拟合实验数据可以为模型B提供可接受的参数;对于模型A,我们得到不可接受的结果。 [参考:20]

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