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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >B-cell responses to myelin basic protein and its epitopes in autoimmune encephalomyelitis induced by Semple rabies vaccine.
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B-cell responses to myelin basic protein and its epitopes in autoimmune encephalomyelitis induced by Semple rabies vaccine.

机译：Semple狂犬病疫苗诱导的自身免疫性脑脊髓炎中B细胞对髓鞘碱性蛋白及其表位的反应。

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摘要

Semple rabies vaccine is composed of rabies virus-infected sheep or goat brain inactivated with phenol and is administered daily after exposure for 14-21 days. Semple rabies vaccine-induced autoimmune encephalomyelitis (SAE) has clinico-pathological findings of demyelination similar to experimental autoimmune encephalomyelitis (EAE) caused by injection of central nervous system tissue or purified myelin proteins into experimental animals and frequently studied as a model for the human demyelinating disease, multiple sclerosis (MS). T-cell-mediated immune responses play a major role in induction of EAE, and antibody responses enhance disease severity. We studied the antibody responses to myelin basic protein (MBP) in 24 Thai patients with SAE and 77 control individuals to define the linear epitopes in human MBP that are encephalitogenic. Antibody levels were assessed by ELISA using native human MBP or synthetic MBP peptides of 20 amino acids. The major B-cell epitope was MBP61-80 and a minor epitope was MBP106-140 in SAE while in MS the major B-cell epitope is MBP84-96. MBP61-80-specific IgG1 and IgG3 levels were significantly higher in patients than controls while IgG2 and IgG4 were not. The data support the hypothesis that autoreactive Th1 cells induce SAE. The difference in B-cell epitope recognition may be due to differences in the genetic backgrounds of the populations studied or may reflect underlying differences in the pathogenesis of SAE and MS.
机译：半狂犬病疫苗由用酚灭活的狂犬病病毒感染的绵羊或山羊脑组成，在暴露14-21天后每天给药。狂犬病疫苗诱导的自身免疫性脑脊髓炎（SAE）具有与实验性自身免疫性脑脊髓炎（EAE）类似的脱髓鞘的临床病理学发现，这种现象是由向实验动物注射中枢神经系统组织或纯化的髓鞘蛋白引起的，并且经常作为人脱髓鞘的模型进行研究疾病，多发性硬化症（MS）。 T细胞介导的免疫反应在EAE的诱导中起主要作用，而抗体反应则增强了疾病的严重程度。我们研究了24名泰国SAE患者和77名对照个体对髓鞘碱性蛋白（MBP）的抗体反应，以确定人MBP中具有致脑病性的线性表位。使用天然人MBP或20个氨基酸的合成MBP肽，通过ELISA评估抗体水平。在SAE中，主要的B细胞抗原决定簇是MBP61-80，次要的抗原决定簇是MBP106-140，而在MS中主要的B细胞抗原决定簇是MBP84-96。患者的MBP61-80特异性IgG1和IgG3水平明显高于对照组，而IgG2和IgG4则没有。数据支持自反应性Th1细胞诱导SAE的假设。 B细胞表位识别的差异可能是由于研究人群的遗传背景不同，也可能反映了SAE和MS发病机理的根本差异。

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