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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Lymphocytes delay kinetics of HSV-1 reactivation from in vitro explants of latent infected trigeminal ganglia.
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Lymphocytes delay kinetics of HSV-1 reactivation from in vitro explants of latent infected trigeminal ganglia.

机译：淋巴细胞延迟了潜在感染的三叉神经节的体外外植体中HSV-1激活的动力学。

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摘要

A persistent immune response to herpes simplex virus type 1 (HSV-1) is evidenced by the expression of cytokine transcripts along with infiltrating mononuclear cells in the ganglia of latently infected mice. In trigeminal ganglion (TG) explant co-cultures, the presence of nonirradiated or irradiated primed splenocytes significantly reduced HSV-1 reactivation as defined by secreted infectious HSV-1 found in the supernatants of TG explant cultures. Primed splenocytes depleted of CD4+ or CD8+ cells did not antagonize HSV-1 reactivation. Cytokines including interleukin (IL)-2, IL-6, IL-10, and IL-12 were all detected in the TG explant cultures containing splenocytes. To further study the role of cytokines in HSV-1 reactivation, dissociated TG cell cultures were treated with exogenous recombinant cytokines including IFN-alpha or -gamma, IL-4, 6, 10, 12 or tumor necrosis factor (TNF)-alpha at concentrations ranging from 2.0 pg to 2.0 ng/culture (or 0.3-300 units/culture for the IFNs). While no cytokines tested at any concentration significantly modified HSV-1 reactivation, neutralizing antibody to IL-6, but not to IFN-alpha/beta, significantly antagonized HSV-1 reactivation. Collectively, the results suggest that IL-6 is directly or indirectly involved in HSV-1 reactivation in TG explant cultures.
机译：潜伏感染小鼠神经节中细胞因子转录物和浸润的单核细胞的表达证明了对1型单纯疱疹病毒（HSV-1）的持续免疫应答。在三叉神经节（TG）外植体共培养物中，未辐照或经辐照的脾细胞的存在显着降低了HSV-1的再激活，如在TG外植体培养物上清液中发现的分泌型传染性HSV-1所定义的。耗尽了CD4 +或CD8 +细胞的初免脾细胞不会拮抗HSV-1的激活。在含有脾细胞的TG外植体培养物中均检测到包括白介素（IL）-2，IL-6，IL-10和IL-12在内的细胞因子。为了进一步研究细胞因子在HSV-1激活中的作用，用IFN-α或-γ，IL-4、6、10、12或肿瘤坏死因子（TNF）-α等外源重组细胞因子处理了分离的TG细胞培养物。浓度范围为2.0 pg至2.0 ng /培养物（或IFNs为0.3-300单位/培养物）。尽管没有测试任何浓度的细胞因子都能显着改变HSV-1的活化，但中和针对IL-6而不是针对IFN-α/β的抗体会明显拮抗HSV-1的活化。总体而言，结果表明IL-6直接或间接参与TG外植体培养物中HSV-1的活化。

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